Development Stage | Treatment Group | n | Mean bacterial load | +/- SD | Median bacterial load | p* |
|---|
Lavae | Untreated | 5 | 2.8 × 104 | 2.4 × 104 | 1.7 × 104 |  |
|  | 106 CFU/mg | 12 | 3.9 × 104 | 3.3 × 104 | 2.6 × 104 |  |
|  | 107 CFU/mg | 5 | 2.3 × 105 | 1.8 × 105 | 2.5 × 105 | 0.055 |
Pupae | Untreated | 9 | 2.0 × 104 | 1.9 × 104 | 1.3 × 104 |  |
|  | 106 CFU/mg | 12 | 6.0 × 104 | 3.6 × 104 | 4.6 × 104 |  |
|  | 107 CFU/mg | 12 | 3.3 × 105 | 2.5 × 105 | 2.6 × 105 | <0.0001 |
Sand fly | Untreated | 4 | 1.5 × 104 | 1.4 × 104 | 1.2 × 104 |  |
|  | 106 CFU/mg | 12 | 2.2 × 104 | 2.0 × 104 | 1.7 × 104 |  |
|  | 107 CFU/mg | 12 | 3.9 × 104 | 3.8 × 104 | 2.3 × 104 | 0.32 |
- • Kruskal-Wallis test
- The Kruskal-Wallis test indicated a slight difference in the bacterial load in larvae (p = 0.055) and a significant difference in pupae (p<0.0001). No significant difference was observed in bacterial load in emergent sand flies (p = 0.32). The location and identification of intergroup differences were performed using the Mann-Whitney test. In larvae, there was no difference in bacterial load between insects that developed in untreated chow and chow treated with 106 CFU/mg of B. subtilis (p = 0.60), or between insects from untreated chow and chow treated with 107 CFU/mg of B. subtilis (p = 0.055). However, statistical difference was observed in bacterial load between larvae from chow treated with 106 CFU/mg and 107 CFU/mg of B. subtilis (p = 0.035). Statistical difference were observed in pupae from untreated chow and chow treated with 106 CFU/mg of B. subtilis (p = 0.0062), pupae from untreated chow and chow treated with 107 CFU/mg of B. subtilis (p < 0.0001) and pupae from chow treated with 106 and 107 CFU/mg of B. subtilis (p < 0.0001).
