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Table 1 Probes and primers used in this study

From: Molecular identification of Clonorchis sinensis and discrimination with other opisthorchid liver fluke species using multiple Ligation-depended Probe Amplification (MLPA)

Primer

Oligonucleotides Sequences(5`-3`)

CsPLaw

PaACATACATGTAGAACATGACGG GTTCGGGCAATTCGTTATTGGCCCTATAGTGAGGTCTTCTCTATTGTCACCGT ATGCACATCTCGGAATCAAGCTGg

CsPLfw

hACTGGATTCAGGTTCACGA AGCTGCATTATCGATCAGTACCAGTGTAGTACAGCGCCGGTGAAATTATCGCCACAGGCCTTTCTGCAGCATGCATGCGGAb

OfPLaw

PcCTATACATGTAGAATATGACGGA GTTCGGGCAATTCGTTATTGGCCCTATAGTGAGGTCTTCTCTATTGTCACCGTATGCACATCTCGGAATCAAGCTGg

OfPLfw

hACTGGATTCAGGTTCACGA AGCTGCATTATCGATCAGTTATCGCCACAGGCCTTTAACGCTGCAGCATGTATGTd

OvPLaw

PeTTATACATGTAGGTTATACATGAC GTTCGGGCAATTCGTTATTGGCCCTATAGTGAGGTCTTCTCTATTGTCACCGT ATGCACATCTCGGAATCAAGCTGg

OvPLfw

hACTGGATTCAGGTTCACGA AGCTCAGGCCTTTAACGCTGCAGCATGTATGGf

pF3

Fam-CAGCTTAGTTCCGAGATGT

pB3

ACTGGATTCAGGTTCACGA

Cs-T

CCGTCATGTTCTACATGTATGTTCCGCATGCATGCTGCAG

Of-T

TCCGTCATATTCTACATGTATAGACATACATGCTGCAGCGTT

Ov-T

GTCATGTATAACCTACATGTATAACCATACATGCTGCAGCGTT

ITS1f

CGATTCTAGTTCCGTCATCT

ITS1r

CCGCTCAGAGTTGTACTCAT

  1. a-f: complementary sequence of ITS loci in padlock probes, denoted with italics and bold.
  2. g: pF3 binding sequence in underline and bold.
  3. h: pB3 binding sequence in underline.
  4. P: phosphorylated
  5. Fam: Fam fluorescent labled at the 5' end.
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ISSN: 1756-3305

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