Figure 1From: SYBR Green-based Real-Time PCR targeting kinetoplast DNA can be used to discriminate between the main etiologic agents of Brazilian cutaneous and visceral leishmaniasesMelting curve analyses of kDNA conserved regions amplicons. Representative SYBR Green dissociation curves of kDNA amplified products to determinate the melting temperature values [Tm]. Following amplification in the ABI Prism 7500 Sequence Detection System, the amplicons were submitted to a gradual temperature increase (0.1°C/s to 90°C). The Tm calculation of each amplicon was done directly by the software provided (indicated by the perpendicular line in the graphics). Each trace in the charts represents a single analyzed DNA sample. The graphics on the left represent typical kinetic dissociation profiles obtained for the Viannia subgenus: (A1) L. (V.) shawi and L. (V.) braziliensis promastigote reference strains; (B1) clinical samples from cutaneous disease patients; (C1) Lu. intermedia and Lu. migonei pools naturally infected by L. (V.) braziliensis. On the right are represented the melting profiles for the subgenus Leishmania: (A2) L. (L.) amazonensis and L. (L.) infantum promastigote reference strains; (B2) clinical specimens from visceral disease patients; (C2) Lu. cruzi and Lu. forattinii pools naturally infected by L. (L.) infantum.Back to article page