Figure 2
From: Detection of dengue group viruses by fluorescence in situ hybridization
Diagnostic PCR of viral RNA in C6/36 cells. C6/36 cells were infected with DENV-1 to DENV-4, YFV or WNV and 5 days later RNA was extracted from cells and reverse transcribed. PCR was performed on the cDNA obtained to amplify a 230-bp fragment of the NS5 gene, conserved among Flavivirus. Fragments of the expected size were obtained for all the six viruses, but not for uninfected cells. Ladder Mass Ruler DNA Low Range (Fermentas) was used as size marker (bp).