Expression of trypsin modulating oostatic factor (TMOF) in an entomopathogenic fungus increases its virulence towards Anopheles gambiae and reduces fecundity in the target mosquito

Table 1 LD ₅₀ and LT ₅₀ values of wild type and Aea -TMOF expressing Bb -Aa1 strain against Anopheles gambiae

LD₅₀ and LT₅₀ values of wild-type and TMOF expressing *B. bassiana* infections in *Anopheles gambiae*
Strain	Mosquito stage	LD₅₀ (conidia/ml)	LT₅₀ (h)
Wild-type B. bassiana (sf)	Adult	18.34 ± 2.86 x 10^7a	133.26 ± 12.79^b
Bb: spAeaTMOF (sf)	Adult	11.08 ± 2.79 x 10^7a (P=0.01)	113.23 ± 17.56^b (P=0.009)
Wild-type B. bassiana (bf)	Adult	18.00 ± 2.01 x 10^7c	115.04 ± 11.26^d
Bb: spAeaTMOF (bf)	Adult	11.31 ± 0.01 x 10^7c (P= 0.04)	86.48 ± 4.68^d (P=0.01)
Wild-type B. bassiana	Larvae	14.07 ± 1.24 x 10^7e	116.31 ± 13.69^f
Bb: spAeaTMOF	Larvae	11.32 ± 0.77 x 10^7e (P=0.01)	85.05 ± 14.66^f (P=0.001)

^a,c LD₅₀ calculated from the 76 h time point.
^e LD₅₀ calculated from the 48 h time point using the Probit analysis.
^b,d Bioassays performed by spraying mosquitoes with a concentration of 1x10⁸ spores/ml.
^f Larval bioassays performed using a concentration of 5x10⁶ spores/ml.
Statistical analysis was performed used the Student’s T-test and values were considered significant if P<0.05.

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