Significant variance in genetic diversity among populations of Schistosoma haematobium detected using microsatellite DNA loci from a genome-wide database

Table 2 Details for 15 polymorphic microsatellite loci developed for Schistosoma haematobium

Locus	Primer Sequence 5′ -- > 3′	Repeat motif	T_A	Size (bp)	N	k
Shae_01	F: †GCATCCAATTTCGTACAC	AAT	TD60	257 - 307	65	12
	R: *CCACATTAGGCCAACAAG
Shae_02	F: *TTAGTGTGTTTGGCTTCAAC	AAT	TD60	183 – 225	69	9
	R: †CCTCGAATGAAATCCTGAC
Shae_03	F: †GCTGAGCTTGAGATTG	AAT	TD55	291 – 334	62	15
	R: *CTTCTGTCCCATCGATACC
Shae_04	F: †CCCATCGCTGATATTAAAG	AAT	TD65	289 – 325	70	10
	R: *TCTAGTCGTCTTGGGATCC
Shae_05	F: *TGTGCACAAGAAAGATTAAATG	AAT	TD65	288 – 319	68	10
	R: †ACGACAATGTTGCAAGTTC
Shae_06	F: †GGTGGATTACGCAATAG	AC	TD65	333 – 347	67	8
	R: *TTTAATCAACCGGGTGTC
Shae_07	F: *TCCAAGCACCATTATCAAG	AAT	TD65	315 – 330	66	6
	R: †ACGGAAACTTGTTGAAATG
Shae_08	F: *CTAAACTGGCAAGATTTC	AAT	TD65	299 – 330	68	11
	R: †CAACGTGCCTTTATTTC
Shae_09	F: †GGGATGTATGCAGACTTG	AAT	TD65	213 – 256	68	13
	R: *TTGTTTGGCTGCAGTAAC
Shae_10	F: †CGCATGTCATACCTATCTCC	AAT	TD65	198 – 213	69	6
	R: *GCTTATCAGGCCTATCTCC
Shae_11	F: *TTGGTTTAGAAATTACATCACC	ATC	TD65	207 – 222	66	5
	R: †CCAACAATATTAATGGACAGC
Shae_12	F: †CGTCTTAGTGAGCCAGATG	AAC	TD65	257 – 278	68	6
	R: *CTCGTGGACATCATCAG
Shae_13	F: *GAGCAGCTATTTCGTATCG	AAT	TD60	183 – 225	69	12
	R: †ACCGTGGACAGTTCATCAG
Shae_14	F: *GTCCTCCTTCCCTCTTTG	ACTC	TD65	210 – 254	67	10
	R: †CACATTCGTCCTAGATATCG
Shae_15	F: *CTTTCAGTAGGATTTGTTG	ATC	TD65	300 – 322	67	9
	R: †CGACGTCAAGCACTGTAC

* indicates CAG tag (5′- CAGTCGGGCGTCATCA-3′).
† indicates pigtail (5′- GTTT-3′).
The annealing temperature (T_A) where TD65, TD60, and TD55 indicates touchdown protocols with a highest annealing temperature of 65°C, 60°C, and 55°C, respectively; size indicates the range of sequenced alleles in base pairs plus the length of the CAG tag; the number of individuals genotyped out of 72 is N; k is the number of alleles observed.

ISSN: 1756-3305