Amblyomma cajennense is an intrastadial biological vector of Theileria equi

Parasites & Vectors

Table 6 Intrastadial transmission Experiment 2, adult ticks were acquisition fed on T. equi infected horse Ho-266; males for 14 days (parasitemia averaged 1.19 × 10 ⁵ genome copies per ml of blood over the 11 day acquisition feeding period, as determined by qPCR), females for 11 days (9.67 × 10 ⁴ )

Horse #	Tick species	Sex	Incubation (days)^a	Transmission feed (days)	# recovered/# applied	# gut pos/# tested	# SG pos/# tested
Ho-209	A. caj	♂♂	16	18	2/69	0/2	0/2
		♀♀^b	19	9-18^c	8/51	2/3	0/3
Ho-211	D. var	♂♂	16	18	15/33	0/15	0/15
		♀♀^b	19	6-18^c	60/66	0/15	0/15

After acquisition feeding A. cajennense were placed on Ho-209 and D. variabilis on Ho- 211 for transmission feeding. Ho-209 acquired infection. After transmission feeding all males and a sample of females were dissected and their guts and salivary glands tested for infection by nPCR.
^aTime between acquisition feed and transmission feed.
^bPartially engorged females put on for reattachment.
^cReflects the amount of time required for female ticks to feed to repletion and drop off.

ISSN: 1756-3305