Table 1 Different PCR reactions used in the current study

GPI-PLC-PCR [12]

Trypanozoon

324 bp

657: 5’- CGC TTT GTT GAG GAG CTG CAA GCA-3’

40

95°C for 15 min then 94°C for 30 sec, 63°C for 90 sec, 72°C for 70 sec, 72°C then for 10 min.

658: 5’- TGC CAC CGC AAA GTC GTT ATT TCG-3’

TBR-PCR [9]

Trypanozoon

173 bp

TBR1: 5’-CGA ATG AAT ATT AAA CAA TGC GCA GT-3’

35

94°C for 3 min then 94°C for 1 min, 55°C for 1 min, 72°C for 30 sec, then 72°C for 5 min.

TBR2: 5’-AGA ACC ATT TAT TAG CTT TGT TGC-3’

TCS-PCR [14]

T. congolense savannah

316 bp

TCS1: 5’-CGA GAA CGG GCA CTT TGC GA-3’

35

94°C for 3 min then 94°C for 1 min, 60°C for 2 min, 74°C for 30 s.

TCS2: 5’-GGA CAA ACA AAT CCC GGGCA CA-3’

TCK [14]

T. congolense kilifi

294 bp

TCK1: 5’- GTG CCC AAA TTT GAA GTG AT-3’

35

94°C for 3 min then 94°C for 1 min, 60°C for 2 min, 74°C for 30 s.

TCK2: 5’- ACT CAA AAT CGT GCA CCT CG-3’

TCF [14]

T. congolense forest

350 bp

TCF: 5’- GGA CAC GCC AGA AGG TAC TT-3’

35

94°C for 3 min then 94°C for 1 min, 60°C for 2 min, 74°C for 30 s.

TCF2: 5’- GTT CTC GCA CCA AAT CCA AC-3’

TV-PCR [16]

T. vivax

400 bp

ILO1264: 5’-CAG CTC GGC GAA GGC CAC TTC GCT GGG GTG-3’

35

94°C for 90 s min, 55°C for 90 s, 72°C for 2 min.

ILO1265: 5’-TCG CTA CCA CAG TCG CAA TCG TCG TCT CAA GG-3’

West African TV-PCR [15]

T. vivax

175 bp

TVW1: 5’-GTG CTC CAT GTG CCA CGT TG-3’

35

94°C for 1 min, 55°C for 1 min, 72°C for 2 min.

TVW2: 5’-CAT ATG GTC TGG GAG CGG GT-3’

ITS-PCR [20]

T. congolense forest

1513 bp

ITS1: 5'-GAT TAC GTC CCT GCC ATT TG-3'

Two rounds, 35 cycles, each, the second round PCR was seeded with 1 μl from the first round

95°C for 7 minutes then 94°C for 1 min, 55°C for 1 min, 72°C for 2 minutes.

T. congolense kilifi

1422 bp

T. congolense savannah

1413 bp

ITS2: 5'-TTG TTC GCT ATC GGT CTT CC-3'

T. brucei s.l.

1207-1224 bp

ITS3: 5'-GGA AGC AAA AGT CGT AAC AAG G-3'

T. theileri

988 bp

ITS4: 5'-TGT TTT CTT TTC CTC CGC TG-3'

T. simiae Tsavo

954 bp

T. simiae

850 bp

T. vivax

611 bp