| Primer/Probe | 5′-3′ nucleotide sequence | |
---|---|---|---|
Duplex screening assay | |||
 |  | Bb23Sf | CGAGTCTTAAAAGGGCGATTTAGT |
B. burgdorferi | 23S rRNA | Bb23Sr | GCTTCAGCCTGGCCATAAATAG |
 |  | Bb23S-P | FAM-AGATGTGGTAGACCCGAAGCCGAGTG-TAMRA |
 |  | ApMSP2f | ATGGAAGGTAGTGTTGGTTATGGTATT |
A. phagocytophilum | msp2 | ApMSP2r | TTGGTCTTGAAGCGCTCGTA |
 |  | ApMSP2-P | VIC-TGGTGCCAGGGTTGAGCTTGAGATTG-TAMRA |
B. burgdorferi confirmatory assay(s)* | |||
 |  | ospAF | CTGGGGAAGTTTCAGTTGAAC |
B. burgdorferi | ospA | ospAR | TTGGTGCCATTTGAGTCGTA |
 |  | ospA-P | FAM-CTGCAGCTTGGAATTCAGGCACTT-BBQ |
 |  | BbIGSf | AAGAAGGACAAGTATTGTAGCGAG |
B. burgdorferi | IGS* | BbIGSr | GCAATCTTTGCCTTCCTCC |
 |  | BbIGS-P | FAM-TGCCAGTATTTAGTGGTAGGGATTCGG-BBQ |
B. miyamotoi assays | Â | Â | Â |
 |  | BmiyaIGSf | CGTCTTGTTGCTTTTAAAGTGT |
B. miyamotoi | IGS | BmiyaIGSr | CATGATCAGGTCCTTGATAATATG |
 |  | BmiyaIGS-P | FAM-TGGATTCCAAATTTGATTACATGCAA-BBQ |
 |  | MGlpQF | GATAATATTCCTGTTATAATGC |
B. miyamotoi | glpq | MGlpQR | CACTGAGATTTAGTGATTTAAGTTC |
 |  | MYS-P | FAM-CCCAGAAATTGACAACCACAAATGT-BHQ2 |
Borrelia spp. Nested PCR | Â | Â | Â |
16S-23S IGS | 1st stage | rrs | GTATGTTTAGTGAGGGGGGTG |
 |  | rrl | GGATCATAGCTCAGGTGGTTAG |
 | 2nd stage | Fn | AGGGGGGTGAAGTCGTAACAAG |
 |  | Rn | GTCTGATAAACCTGAGGTCGGA |
Flagellin | 1st stage | FO1 | AAGTAGAAAAAGTCTTAGTAAGAATGAAGGA |
 |  | FO2 | AATTGCATACTCAGTACTATTCTTTATAGAT |
 | 2nd stage | FI1 | CACATATTCAGATGCAGACAGAGGTTCTA |
 |  | FI2 | GAAGGTGCTGTAGCAGGTGCTGGCTGT |