Table 1 Primers/Probes used for RmAQP1 transcript cloning and real-time PCR
Primer ID | Sequence | Use |
|---|---|---|
KB-126 | 5′ GAGCGGGCACATGCAGTTGTAGGC 3′ | Reverse for Aquaporin 5′ RACE |
KB-156 | 5′ ACTCAGGAATTC ATGAAGATCGAGAACCT 3′ | Forward for insertion into pPICZ αA EcoRI |
KB-157 | 5′ TCACTGGCGGCCGC CGGGCACATGCAGTTGTAGGC 3′ | Reverse for insertion into pPICZ αA NotI |
KB-238 | 5′ TCGCCAAAGTGCCGCTATAC 3′ | Aquaporin RT-PCR Forward |
KB-239 | 5′ CGTCTTTGTAGGTGGCAAACAC 3′ | Aquaporin RT-PCR Reverse |
KB-240 | 5′ 6FAM-CGCCGCACCGACGAAGCCAC-TAMRA 3′ | Aquaporin TaqMan Probe |
KB-263 | 5′ TAAGGACCTGTACGCCAACAC 3′ | Beta-actin RT-PCR Forward |
KB-264 | 5′ CGGTGATTTCCTTCTGCATACG 3′ | Beta-actin RT-PCR Reverse |
KB-265 | 5′ 6FAM-TCTCCGGCGGCACCACCATGTACC-TAMRA 3′ | Beta-actin TaqMan Probe |
AF018656-363 F | 5′ CCTGAGAAACGGCTACCACATC 3′ | 18S rRNA RT-PCR Forward |
AF018656-425R | 5′ GTGCCGGGAGTGGGTAATT 3′ | 18S rRNA RT-PCR Reverse |
AF018656-387 | 5′ 6FAM-AGGAAGGCAGCAGGCGCGC-TAMRA 3′ | 18S rRNA TaqMan Probe |