Table 1 Primer targets, sequences and amplicon base pair size for previously unreported quantitative PCR assays used in this study. F: Forward primer; R: reverse primer
Target | Primers | Amplicon (bp) |
|---|---|---|
Anaplasma -tr-1 | F- 5′ ATGTTTATGACTTCTCAAGCAC-3′ R- 5′ CCC TTT TCG TAT TTT TGT AC-3′ | 200 |
A. phagocytophilum -p44 | F- 5′ TGGTGGTGCGGGATATTTCTATGTTG-3′ R- 5′ CCCAATCCGAGGATCAGGTGTG-3′ | 179 |
A. platys -p44 | F- 5′ GCT AAG TGG AGC GGT GGC GAT GAC AG-3′ R- 5′ GCCGCAGTTTCCCCGGTACT-3′ | 179 |
E. chaffeensis -nadA | F- 5′ CGCAAAAGATGTAATTCTTTGGGAATC-3′ R- 5′ CACCTCAAAATCAGAATTCATCGAAGG-3′ | 117 |
E. ewingii -sodB | F- 5′ GCTGGAATAGGTCATTTTGGTAGTGGA-3′ R- 5′ GTTCCCATACATCCATAGCAAGCAAC-3′ | 147 |