Fig. 2

Immunocytochemical localisation of calmodulin 3 (FhCaM3) in juvenile Fasciola hepatica. Green immunoreactivity (IR) represents FhCaM3 labelled with fluorescein isothiocyanate, red represents filamentous actin labelled with phalloidin-tetramethylrhodamine isothiocyanate. Images a–d represent consecutive optical sections taken in the Z-axis (at 1.5 μm intervals), demonstrating the presence of abundant FhCaM3 immunoreactivity (IR) throughout the sub-tegumental, sub-muscular parenchyma. The bifurcated gut (g) can be seen in b–d. e–f show higher magnification images in which FhCaM3 IR is visible within cell bodies (cb) and cellular processes (p), while nuclei (n) remain un-stained. Identical staining patterns were observed for FhCaM2 (not shown). These patterns were visualised in 30 samples