Molecular detection of tick-borne bacteria and protozoa in cervids and wild boars from Portugal

Parasites & Vectors

Table 1 Sequences of the oligonucleotide primers used

Pathogen	Target gene		Oligonucleotide sequences (5′-3′)		Amplicon size (bp)	Reference
			Forward	Reverse
Anaplasma spp./Ehrlichia spp.	16S rRNA		GGTACCYACAGAAGAAGTCC	TAGCACTCATCGTTTACAGC	345	[38]
Anaplasma spp./Ehrlichia spp.	groEL		ACTGATGGTATGCARTTTGAYCG	TCTTTRCGTTCYTTMACYTCAACTTC	600	[39]
Anaplasma marginale/A. centrale/A. ovis	msp4		GGGAGCTCCTATGAATTACAGAGAATTGTTTAC	CCGGATCCTTAGCTGAACAGGAATCTTGC	851	[40]
Anaplasma phagocytophilum	msp4		ATGAATTACAGAGAATTGCTTGTAGG	TTAATTGAAAGCAAATCTTGCTCCTATG	849	[13]
Babesia spp./Theileria spp.	18S rRNA		AATACCCAATCCTGACACAGGG	TTAAATACGAATGCCCCCAAC	400	[38]
Borrelia burgdorferi (sensu lato)	ITS 5S-23S rRNA	Outer primmers	ACCATAGACTCTTATTACTTTGAC	TAAGCTGACTAATACTAATTACCC	380	[41]
		Inner primers	ACCATAGACTCTTATTACTTTGACCA	GAGAGTAGGTTATTGCCAGGG	225	[41]
	flaB	Outer primmers	TGGTATGGGAGTTTCTGG	TAAGCTGACTAATACTAATTACCC	774	[42]
		Inner primers	CAGACAACAGAGGGAAAT	TCAAGTCTATTTTGGAAAGCACC	604
Rickettsia spp.	gtlA		GGGGGCCTGCTCACGGCGG	ATTGCAAAAAGTACAGTGAACA	381	[43]

ISSN: 1756-3305