Table 1 Primers used in all experiments
Primer ID and direction | Primer sequence 5′–3′ |
|---|---|
Hc-daf-22 1F | TACAGACAGTGCCCGGCCATCA |
Hc-daf-22 1R | GGCAGTAATAGGGGCAGGGAACA |
Hc-daf-22 2F | GCTTCTGGCTCTTCTGGGCTCTACT |
Hc-daf-22 2R | GAAAGCGGTAACTCTGACGTTGTGCTTGC |
Hc-daf-22 F1 | ATGGGTAAATCAAAAGTATATG |
Hc-daf-22 R1 | ACTGAGTGCAGATGGTT |
Hc-daf-22 F2 | ACGCCGGCAAAGAACATAT |
Hc-daf-22 R2 | CCCTTACCGACGTCACACACAAT |
Hc-daf-22 F3 | ACTTATGAGGCTCTGGGATTG |
Hc-daf-22 R3 | GCATCGATAGTCCACTTCTTTAC |
Hc-daf-22 F4 | AGGAAAATGGGTGATCAATCCGTCT |
Hc-daf-22 R4 | TCAGATTTTCGCTTTGAGCATTTTC |
Hc-daf-22-SP1 | GATAGGTATGCCAGTGAACCCGAGT |
Hc-daf-22-SP2 | GCAATCGTCCAACGCCATATTCACT |
Hc-daf-22-SP3 | AATACACTCACCGGCTTCCTTCACC |
Hc-daf-22 RT F | GTATATGTGATTGGTGTCGG |
Hc-daf-22 RT R | AATATCCGACGGTAGCTT |
tub-F | TGTTCCATCACCCAAGGTATCC |
tub-R | TGACAGACACAAGGTGGTTGAGAT |
Hc-daf-22PF | AACTGCAGGATTGGTCGAGAGAGATGAAAC |
Hc-daf-22PR | CGGGATCCGATGGTCCGGGCTGCAATAAATT |
Hc-daf-22dF | TCCCCCGGGATGGGTAAATCAAAAGTATATG |
Hc-daf-22dR | GGGGTACCCAGATTTTCGCTTTGAGCAT |
Ce-PF | GCGCTCTAGATTAATTCAATGCACAACTC |
Ce-PR | TCCCCCGGGTTTCTGGAACAATATT |
RNAi F | CCCAAGCTTGTGAACAATGCCTGCCTGCGCTT |
RNAi R | CTAGCTAGCCCTGTCGCACCGATGGG |