Table 1 List of primers used for 16S rRNA amplification and sequencing
From: Use of MALDI-TOF MS and culturomics to identify mosquitoes and their midgut microbiota
Primers names | Primer sequences (5′–3′) | Temperature (°C) |
|---|---|---|
fd1 | AGA GTT TGA TCC TGG CTC AG | 52 |
rp2 | ACG GCT ACC TTG TTA CGA CTT | 52 |
536 F | CAG CAG CCG CGG TAA TAC | 50 |
536R | GTA TTA CCG CGG CTG CTG | 50 |
800 F | ATT AGA TAC CCT GGT AG | 50 |
800R | CTA CCA GGG TAT CTA AT | 50 |
1050 F | TGT CGT CAG CTC GTG | 50 |
1050R | CAC GAG CTG ACG ACA | 50 |