Table 1 Primer sequences used to amplify the GNBPB2 gene (NCBI Probe database accession number: Pr032290638)
PCR reaction | Primer | Sequence 5′-3′ | Product size (bp) |
|---|---|---|---|
1 | GNBPB2-out-F | CACTCCAGCGAACATTTGTG | 1902 |
GNBPB2-out-R | CTTCAGTGTGTGGCGGTTTA | ||
2 | GNBPB2-in-3-F | CCCTAAATAAAGCGGCACAC | 851 |
GNBPB2-in-3-R | GCACTCTTGATGGGGTTGAT | ||
3 | GNBPB2-in-5-F | GTTCTGGGGATGTGAGCGTA | 963 |
GNBPB2-in-5-R | CAGGGATCTTTTGCGTGATT | ||
4 | GNBPB2-centre-F | ACRGGAGAGCTGATCTTTGA | 571 |
GNBPB2-centre -R | GCCWCGRTAGTCCATATTGC |