Skip to main content

Table 3 Blood meal identification in questing nymphs from EXTF and PATF in the Province of Trento, Italy

From: Identification of Ixodes ricinus blood meals using an automated protocol with high resolution melting analysis (HRMA) reveals the importance of domestic dogs as larval tick hosts in Italian alpine forests

Larval hosts targeted

(primer set used)a

Number and percentage of identified hosts in questing nymphs

Forest type

Total (%)h

EXTF (%)h

PATF (%)h

Rodentia (Muroidea)

46 (31.7)

23 (24.5)

69 (28.9)

  Apodemus spp.

44 (30.3)

23 (24.5)

67 (28.0)

  Myodes glareolus

1 (0.69)

0

1 (0.4)

  Mus musculus

0

0

0

  Cricetus griseus b

1 (0.69)

0

1 (0.4)

Carnivora (Canidae)

33 (22.7)

35 (37.2)

68 (28.4)

  Canis lupus familiaris

28 (19.3)

23 (24.5)

51 (21.3)

  Vulpes vulpes

5 (3.4)

12 (12.7)

17 (7.1)

Cetartiodactyla (Caprinae, Cervidae)

30 (20.7)

11 (11.7)

41 (17.2)

  Ovis spp.c,d

5 (3.4)

2 (2.1)

7 (2.9)

  Rupicapra rupicapra d

3 (2.1)

0

3 (1.3)

  Capra hircus c

2 (1.4)

0

2 (0.8)

  Bos taurus c

0

3 (3.2)

3 (1.3)

  Capreolus capreolus d

8 (5.5)

2 (2.1)

10 (4.2)

  Cervus elaphus d

12 (8.3)

3 (3.2)

15 (6.3)

  Dama dama b,d

0

1 (1.1)

1 (0.4)

Passeriformes (Passeriformes)

14 (9.6)

21 (22.3)

35 (14.6)

  Turdus merula

8 (5.5)

8 (8.5)

16 (6.7)

  Turdus spp./Erithacus rubecula e

6 (4.1)

13 (13.8)

19 (7.9)

Soricomorpha (Soricidae)

22 (15.2)

4 (4.3)

26 (10.9)

  Sorex spp.

9 (6.2)

0

9 (3.8)

  Sorex araneus

0

0

0

  Crocidura leucodon

7 (4.8)

2 (2.1)

9 (3.8)

  Crocidura suaveolens

6 (4.1)

2 (2.1)

8 (3.3)

  Crocidura russula

0

0

0

Total no. of nymphs analysed

506

342

848

Total no. of nymphs with identified blood meal

130

85

215

Total no. of hosts identified

145

94

239

% identification successf

25.7

24.9

25.4

No. of mixed blood meals identified

14

9

23

% of mixed blood mealsg

10.8

10.6

10.7

  1. Abbreviations: EXTF extensive forest, PATF patchy forest
  2. aHost group primers as described in Collini et al. [11]
  3. bHost not targeted in original protocol as described by Collini et al. [11], but identified here after HRMA, by sequencing and BLASTing
  4. cAmplified with Caprinae primers
  5. dAmplified with Cervidae primers
  6. eHRMA of Passeriformes amplicons did not allow discrimination between T. philomelos and E. rubecula
  7. fTotal no. of nymphs with identified blood meal/total no. of nymphs analysed * 100
  8. gTotal no. of mixed blood meals identified/total no. of nymphs with identified blood meal * 100
  9. hNo. of identified host for taxonomic level/total no. hosts identified * 100