Fig. 3

Analysis of putatively positive clones. a Gel electrophoresis of PCR products amplified from putatively positive prey plasmids. Lane M: DL5000 DNA Marker; Lanes 1–8: eight blue clones containing prey1–8 (prey plasmid isolated from eight blue clones), respectively, were amplified by T7 sequencing primer and 3'AD sequencing primer. b Confirmation of putatively positive clones; C1–C8: zygotes formed from Y187 cells containing Prey1–8 mating with Y2HGold containing empty pGBKT7 vector, respectively. 1–8: zygotes formed from Y187 cells containing Prey1-8 mating with Y2HGold containing pGBKT7-GRA15 bait plasmid, respectively. Control, Y2HGold transformed with pGBKT7-53 or empty pGBKT7 vector were used to mate with Y187 cells containing pGADT7-T, respectively. The mated cultures were then grown on QDO/X/A agar plates, respectively