Microbiota activates IMD pathway and limits Sindbis infection in Aedes aegypti

Parasites & Vectors

Table 1 List of primers used in this study

Accession number	Gene	Primer sequence (5’–3’)
AAEL007696	aaREL1 For	GACTCGTCGGAGCTGAAATC
AAEL007696	aaREL1 Rev	CGGTTTGTTCAGGTTGTTGA
AAEL007624	aaREL2 For	TCTGTCGGCAGATGAAGTGA
AAEL007624	aaREL2 Rev	GCACTGGAATGGAGAATCAAA
AAEL000709	Cactus For	TCTTGCGTTGAAGTGAGTGG
AAEL000709	Cactus Rev	GACCCTCTGAAAGGGAAAGG
AAEL003841	Defensin For	GATTCGGCGTTGGTGATAGT
AAEL003841	Defensin Rev	TTATTCAATTCCGGCAGACG
AAEL010083	IMD For	TCGTCAAACTCGGTTTTCCT
AAEL010083	IMD Rev	TGGCGGAGTTGAAGGTAAAG
AAEL007768	Myd88 For	CGATGCGTTCATTTTGTTTG
AAEL007768	Myd88 Rev	CACCGCTCAGAAATCAGCTT
AAT45939	RP49 For	GCTATGACAAGCTTGCCCCCA
AAT45939	RP49 Rev	TCATCAGCACCTCCAGCT
AAEL005673	Serpin For	ACGTGATGGATTGGATGGAG
AAEL005673	Serpin Rev	GTGCCTGCACTTGTTTCTGA
AAEL009692	STAT For	CACACAAAAAGGACGAAGCA
AAEL009692	STAT Rev	TCCAGTTCCCCTAAAGCTCA
AAEL001794	TEP For	ATTTTTGACGGCTTTTGTGG
AAEL001794	TEP Rev	TGGATTACTTGCCCCACTTC
Sindbis virus quantification primer	SINV For	TGACTAACCGGGGTAGGT
Sindbis virus quantification primer	SINV Rev	TTGGCTTCGGTGGGCATC
AAEL003579	dsCaspar For	TAATACGACTCACTATAGGG* GGAAGCAGATCGAGCCAAGCAG
AAEL003579	dsCaspar Rev	TAATACGACTCACTATAGGG* GCATTGAGCCGCCTGGTGTC
16S (For bacteria quantification)	16S For	TCCTACGGGAGGCAGCAGT
16S (For bacteria quantification)	16S Rev	GGACTACCAGGGTATCTAATCCTGTT
M13 (LacZ fragment)	M13 For	GTAAAACGACGGCCAGT
M13 (LacZ fragment)	M13 Rev	CTCGAGTAATACGACTCACTATAGGGCAGGAAACAGCTATGAC

ISSN: 1756-3305