Table 1 Primers and protocols used for the amplification of Cytauxzoon sp., Hepatozoon spp. and housekeeping GAPDH gene control
PCR primers (5′-3′) | PCR conditions | Product size (bp) | |
|---|---|---|---|
PCR-1 | F: CCAGCAGCCGCGGTAATTC | 94 °C, 3 min; 35 cycles [94 °C, 30 s, 64 °C, 45 s, 72 °C, 30 s]; 72 °C, 7 min | 373 |
R: CTTTCGCAGTAGTTYGTCTTTAACAAATCT | |||
PCR-2 | F: CCTGGTTGATCCTGCCAG | 96 °C, 3 min; 40 cycles [96 °C, 1 min, 65 °C, 1 min, 72 °C, 2 min]; 72 °C, 1 min | 1,675 |
R: CGACTTCTCCTTCCTTTAAG | |||
Housekeeping (GAPDH) | F: CCTTCATTGACCTCAACTACAT | 95 °C, 1 min; 45 cycles [94 °C, 4 s, 57 °C, 4 s, 72 °C, 3 s]; 72 °C, 1 min | 282 |
R: CCAAAGTTGTCATGGATGACC |