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Fig. 1 | Parasites & Vectors

Fig. 1

From: Secreted phospholipase A2 of Clonorchis sinensis activates hepatic stellate cells through a pathway involving JNK signalling

Fig. 1

Prokaryotic expression and purification of protein CssPLA2 with plasmid pMAL-c2X. a PCR product of CssPLA2 (828 bp). Lane M: DNA molecular marker; Lane 1: ddH2O negative control; Lane 2: PCR product of CssPLA2 (828 bp). b SDS-PAGE analysis of the prokaryotic expression product of CssPLA2. Lane M: protein molecular weight marker; Lane 1: CssPLA2/pMAL-c2X transformants without IPTG induction; Lane 2: supernatant of the lysate of bacteria with CssPLA2/pMAL-c2X after IPTG induction; Lane 3: precipitation of the lysate of bacteria with CssPLA2/pMAL-c2X after IPTG induction; Lane 4: purified MBP-CssPLA2 protein. c Anion exchange chromatography for purification of MBP-CssPLA2 protein. d SDS-PAGE analysis of MBP-CssPLA2 protein after anion exchange chromatography. Lane M: protein molecular weight marker; Lane 1: MBP-CssPLA2 protein purified by amylose resin; Lane 2: MBP-CssPLA2 protein purified by amylose resin and anion exchange chromatography

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Parasites & Vectors

ISSN: 1756-3305

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