Fig. 4
JNK signalling was activated in the liver tissue of Balb/C mouse with abdominal injection of MBP-CssPLA2. a Liver sections of five groups of Balb/C mouse with HE and Masson staining. Sections 1 & 2, HE and MASSON staining of liver sections of Balb/C mouse with an abdominal injection of PBS; Sections 3 & 4, HE and MASSON staining of liver sections of Balb/C mouse with an abdominal injection of 100 μg ESPs; Sections 5 & 6, HE and MASSON staining of liver sections of Balb/C mouse with an abdominal injection of 100 μg MBP; Sections 7 & 8: HE and MASSON staining of liver sections of Balb/C mouse with an abdominal injection of 50 μg MBP-CssPLA2; Sections 9 & 10, HE and MASSON staining of liver sections of Balb/C mouse with an abdominal injection of 100 μg MBP-CssPLA2; b Western blot of liver tissue of five groups of Balb/C mouse with P-JNK1 monoclonal antibody. Lane 1: Liver tissue of Balb/C mouse with an abdominal injection of PBS; Lane 2: Liver tissue of Balb/C mouse with an abdominal injection of 100 μg ESPs; Lane 3: Liver tissue of Balb/C mouse with an abdominal injection of 100 μg MBP; Lane 4: Liver tissue of Balb/C mouse with an abdominal injection of 50 μg MBP-CssPLA2; Lane 5: Liver tissue of Balb/C mouse with an abdominal injection of 100 μg MBP-CssPLA2. c Quantitative western blot analysis of P-JNK1. Unpaired t-test was applied for statistical analysis. Compared with control group, JNK signalling of experimental group, the liver tissue of Balb/C mouse with an abdominal injection of 50 μg MBP-CssPLA2 was activated (t (4) = 3.298, P = 0.03)