Fig. 4
From: Characterization of a novel aspartyl protease inhibitor from Haemonchus contortus
Expression patterns of API in different life stages of H. contortus. Expression levels of API mRNA in egg, L3, xL3, adult male and female were tested by real time PCR. The relative quantities (RQ, compared with female adult worm, female = 1) in egg, L3, xL3 and adult male were 4.3, 43.8, 10.5 and 2.6, respectively. The value of API gene in the female was normalized to 1.0. The relative changes in gene expression ratios of selected genes were normalized to the expression of a single reference gene and calculated as described by the 2-ΔΔCt method. Error bars indicate SEM from three independent experiments