Table 2 Primer information for nine microsatellite loci screened in Ae. aegypti from Yunnan Province, China
Locus | Repeat motif | Primer sequence (5′-3′) | Annealing temperature (°C) | Allele size (bp) | Fluorescence labeling | Reference |
|---|---|---|---|---|---|---|
SQM1 | CT10(TT)CT | F: AATCGTGACGCGTCTTTTG; R: TAACTGCATCGAGGGAAACC | 54 | 233–239 | 5′-HEX | [27] |
SQM2 | GA15 | F: CAAACAACGAACTGCTCACG; R: TCGCAATTTCAACAGGTAGG | 53 | 157–183 | 5′-FAM | [27] |
SQM3 | CAT7 | F: ATTGGCGTGAGAACATTTTG; R: GAGGAGTGAGCAGATAGGAGTG | 58 | 156–186 | 5′-FAM | [27] |
SQM4 | TAGA8 | F: GCCAAAAACCAACAAACAGG; R: AATCGACCCGACCAATAACA | 53 | 286–290 | 5′-TAMRA | [27] |
SQM5 | ATA36 | F: GGAGCATTCATAGAGAATTGTCA; R: GAGATGAACCAGTCATAGGGC | 56 | 110–116 | 5′-FAM | [27] |
SQM6 | (TTTA)7(T)14 | F: CGACAGATGGTTACGGACGG; R: GTC CCG CTC CAA AAA TGC CC | 58 | 228 | 5′-FAM | [23] |
SQM7 | AG4 | F: AAAACCTGCGCAACAATCAT; R: AAGGACTCCGTATAATCGCAAC | 56 | 147–169 | 5′-FAM | [36] |
SQM8 | AG5 | F: TGATCTTGAGAAGGCATCCA; R: CGTTATCCTTTCATCACTTGTTTG | 55 | 170–180 | 5′-FAM | [36] |
SQM9 | AC1 | F: TCCGGTGGGTTAAGGATAGA; R: ACTTCACGCTCCAGCAATCT | 55 | 193–209 | 5′-FAM | [36] |