Table 1 Primers and TaqMan probes used in the singleplex and multiplex real-time PCR assays
Species | Target | Primers and probes | Sequence (5′–3′) | Product size (bp) | References |
|---|---|---|---|---|---|
Canis familiaris | cox1 gene | KF/CF-F (forward) | GGGGCTTTGGAAACTGACTA | 95 | Present study |
KF/CF-R (reverse) | TGGAGGAAGGAGTCAGAAGC | ||||
KF/CF-P (probe) | VIC-ATTGGTGCTCCGGACATGGCAT-QSY | ||||
Homo sapiens | cytb gene | KF/HS-F (forward) | CCACCCTCACACGATTCTTT | 104 | Present study |
KF/HS-R (reverse) | GTTGTTTGATCCCGTTTCGT | ||||
KF/HS-P (probe) | NED-TGCAGCCCTAGCAACACTCCACC-NFQ-MGB | ||||
Leishmania spp. | kDNA | LEISH-1 (forward) | AACTTTTCTGGTCCTCCGGGTAG | 120 | [18] |
LEISH-2 (reverse) | ACCCCCAGTTTCCCGCC | ||||
TaqMan-MGB (probe) | FAM-AAAAATGGGTGCAGAAAT-NFQ-MGB |