DNA sample | Quantity (fg/reaction) | Cq value | Mean ± SD | %CV | ||
---|---|---|---|---|---|---|
R1 | R2 | R3 | ||||
Canis familiaris | 108 | 17.27 | 17.27 | 17.01 | 17.18 ± 0.15 | 0.87 |
107 | 19.23 | 19.17 | 19.30 | 19.24 ± 0.06 | 0.33 | |
106 | 22.98 | 22.22 | 21.73 | 22.31 ± 0.63 | 2.82 | |
105 | 25.06 | 25.44 | 26.14 | 25.55 ± 0.55 | 2.15 | |
104 | 28.94 | 29.02 | 28.74 | 28.90 ± 0.15 | 0.51 | |
103 | 33.61 | 32.99 | 34.48 | 33.69 ± 0.75 | 2.22 | |
Homo sapiens | 108 | 16.67 | 16.99 | 16.87 | 16.84 ± 0.16 | 0.96 |
107 | 18.76 | 18.74 | 18.87 | 18.79 ± 0.07 | 0.39 | |
106 | 22.50 | 22.35 | 22.74 | 22.53 ± 0.20 | 0.88 | |
105 | 25.87 | 25.57 | 25.72 | 25.72 ± 0.15 | 0.58 | |
104 | 28.48 | 28.52 | 27.83 | 28.27 ± 0.38 | 1.36 | |
103 | 30.94 | 33.24 | 33.13 | 32.44 ± 1.30 | 4.01 | |
Leishmania spp. | 108 | 5.96 | 5.92 | 6.06 | 5.98 ± 0.07 | 1.23 |
107 | 8.58 | 8.87 | 9.16 | 8.87 ± 0.29 | 3.28 | |
106 | 12.54 | 12.22 | 12.56 | 12.44 ± 0.19 | 1.53 | |
105 | 15.81 | 15.58 | 16.32 | 15.91 ± 0.38 | 2.39 | |
104 | 19.31 | 19.43 | 19.78 | 19.51 ± 0.25 | 1.26 | |
103 | 23.16 | 23.06 | 22.72 | 22.98 ± 0.23 | 1.02 | |
102 | 26.66 | 26.21 | 26.78 | 26.55 ± 0.30 | 1.13 | |
101 | 29.82 | 29.91 | 29.83 | 29.85 ± 0.05 | 0.16 | |
100 | 32.03 | 32.10 | 32.52 | 32.22 ± 0.26 | 0.81 |