Table 4 Inter-assay reproducibility of the multiplex real-time PCR assay

Canis familiaris

10⁸

17.18

17.61

17.29

17.36 ± 0.23

1.30

10⁷

19.24

19.80

19.38

19.47 ± 0.29

1.51

10⁶

22.31

23.30

22.80

22.8 ± 0.50

2.17

10⁵

25.55

26.62

26.18

26.12 ± 0.54

2.07

10⁴

28.90

29.95

29.57

29.47 ± 0.53

1.80

10³

33.69

34.42

33.54

33.88 ± 0.47

1.39

Homo sapiens

10⁸

16.84

15.51

16.43

16.26 ± 0.68

4.19

10⁷

18.79

17.57

18.18

18.18 ± 0.61

3.35

10⁶

22.53

21.30

21.86

21.90 ± 0.62

2.82

10⁵

25.72

24.95

25.33

25.33 ± 0.39

1.53

10⁴

28.27

27.65

27.78

27.90 ± 0.33

1.18

10³

32.44

32.21

31.78

32.14 ± 0.34

1.04

Leishmania spp.

10⁸

5.98

6.76

6.86

6.53 ± 0.48

7.37

10⁷

8.87

9.95

10.24

9.68 ± 0.72

7.44

10⁶

12.44

13.48

13.77

13.23 ± 0.70

5.28

10⁵

15.91

16.91

17.47

16.76 ± 0.79

4.73

10⁴

19.51

20.50

20.87

20.29 ± 0.70

3.47

10³

22.98

24.02

24.84

23.95 ± 0.93

3.89

10²

26.55

27.43

25.47

26.48 ± 0.98

3.71

10¹

29.85

30.40

30.33

30.20 ± 0.30

0.99

10⁰

32.22

32.37

32.79

32.46 ± 0.30

0.92