Fig. 4

Expression of NLR family members, NLRP1, NLRP3, NLRP6, and NLRC4 inflammasome components in response to T. gondii infection in human small intestinal epithelial (FHs 74 Int) cells. a FHs 74 Int cells were infected with T. gondii at a MOI of 10 for 0, 4, and 8 h. Expression of NLRP1, NLRP3, NLRP6, and NLRC4 inflammasome component proteins were detected by western blot analysis; α-tubulin was used as the loading control. b FHs 74 Int cells were fixed and probed against cleaved caspase-8 (green). The cells were counterstained with 4′,6-diamidino-2-phenylindole (blue) and visualized using confocal microscopy. All data shown are representative of three independent experiments with similar results