Fig. 5

In vivo assays with chickens treated with fluralaner by oral gavage. a Experiment 1: chickens treated with 2.5 mg/kg body mass on day 0. b Experiment 2: Chickens treated with 0.5 mg/kg body mass on day 0 and again on day 7. Each experiment consisted of 6 birds. A maximum of 15 bed bugs were fed on each bird at each time point, with each time point therefore represented by 78–87 bed bugs (out of a maximum of 90 bed bugs) that fed to repletion. A linear mixed model (based on restricted maximum likelihood) was conducted within each experiment followed by Tukey’s honestly significant difference test to separate means (represented within box plots by X). Means with different lowercase letters (above box plots) are significantly different at P < 0.05. T1, Treatment 1