Table 1 Primer sets and annealing temperature used in polymerase chain reactions with their respective amplicon size
From: Integrative taxonomy of Metastrongylus spp. in wild boars from Brazil
Region | Primer | T (ºC) | Amplicon | Sequence (5′–3′) | References |
|---|---|---|---|---|---|
18S1 | 988F 1912R | 50 | 1500 bp | CTCAAAGATTAAGCCATGC TTTACGGTCAGAACTAGGG | Holterman [57] |
18S2 | 1813F 2646R | 52 | 1500 bp | CTGCGTGAGAGGTGAAAT GCTACCTTGTTACGACTTTT | Holterman [57] |
28S | D2A D3B | 55 | 650 bp | ACAAGTACCGTGAGGGAAAGTTG TCGGAAGGAACCAGCTACTA | De Ley et al. [58] |
ITS | NC1 NC2 | 54,5 | 800 bp | ACGTCTGGTTCAGGGTTGTT TTAGTTTCTTTTCCTCCGCT | Gasser et al. [59] |
cox-1 | F1 R2 | 45 | 1015 bp | CCTACTATGATTGGTGGTTTTGGTAATTGGTAGCAGCAGTAAAATAAGCACG | Kanzaki and Futai [60] |