Fig. 4From: csi-miR-96-5p delivered by Clonorchis sinensis extracellular vesicles promotes intrahepatic cholangiocarcinoma proliferation and migration via the ferroptosis-related PTEN/SLC7A11/GPX4 axisExtraction and validation of csi-miR-96-5p in CsEVs. a Transmission electron microscopy analysis of CsEVs. b Nanoparticle tracking analysis of the sizes of CsEVs. c Western blot analysis of characteristic CsEV proteins (CD63 and TSG101). d CsEVs labeled with PKH-67 can be taken up by HuCCT1 cells. e qPCR analysis of the level of csi-miR-96-5p in cells treated with CsEVs. CsEVs, Clonorchis sinensis secreted extracellular vesicles; qPCR, quantitative polymerase chain reaction. *P < 0.05, **P < 0.01Back to article page