Species identification of adult ixodid ticks by Raman spectroscopy of their feces

Parasites & Vectors

Table 1 Comparison of vibrational frequency bands for peaks of Raman spectra of fecal samples from 12 species of ixodid ticks

Raman spectral group	Vibrational frequency bands indicating blood (cm⁻¹)	Vibrational frequency bands indicating guanine (cm⁻¹)	Relative intensities of bands 653–1007
Amblyomma (four species)^a	–	653, 852, 940, 1052, 1238, 1268, 1393, 1470, 1553	64.98
Rhipicephalus (Boophilus) (two species)^b	–	653, 852, 940, 1052, 1238, 1268, 1393, 1470, 1553	4.34
Rhipicephalus sanguineus sensu stricto	752, 1007, 1132, 1348, 1376, 1456, 1547, 1623	653, 852, 1238, 1268, 1393, 1425, 1553	1.3
Dermacentor (three species)^c	752, 1007, 1132, 1348, 1376, 1547, 1623	653, 852, 940, 1052, 1238, 1268	0.51
Ixodes scapularis	752, 1007, 1132, 1376, 1456	653, 852, 940, 1238, 1393, 1353	0.94
Haemaphysalis longicornis	752, 1007, 1348, 1376, 1547, 1623	653, 1393, 1553	0.68

^aAmblyomma americanum, Amblyomma maculatum, Amblyomma mixtum, and Amblyomma tenellum
^bRhipicephalus (Boophilus) annulatus and Rhipicephalus (Boophilus) microplus
^cDermacentor albipictus, Dermacentor andersoni, and Dermacentor variabilis

ISSN: 1756-3305