Fig. 5

LDA Effect Size multilevel discriminant analysis of species differences. a Histogram of LDA score distribution shows those biomarkers that were significantly different. The LDA value represents the influence of bacterial species. b Cladogram. The circles radiating from the inside outwards represent taxonomic levels, from kingdom (single circle; innermost) to genus (or species). Each small circle at different classification levels represents a bacterial classification at that level, and diameter of the circle diameter is proportional to the relative abundance. Color coding: The species that are not significantly different are uniformly colored yellow; the species that are significantly different are colored with the group. The red and green nodes represent those bacterial species which play an important role in the red and green groups, respectively: C (red), Control group; T (green), Demodex blepharitis (DB) group. LDA, Linear discriminant analysis