Fig. 5

Maximum likelihood phylogeny [RaxML v.8, bootstrap values > 75 (1000 replicates) are noted at the nodes] of all adult black fly samples successfully barcoded in NM from 2020–2022, 11 voucher specimen sequences and one outgroup, Liohippelates pusio, inferred from mtDNA CoxI. The numbers of total barcodes (excluding voucher sequence) for each Simulium species are listed next to the taxon name, but because of the high number of barcodes every individual was not listed in the phylogeny