Fig. 3
A Fluorescence values of tenfold serial dilutions of Opisthorchis viverrini DNA in Milli-Q sterile water. Results are displayed as mean ± SD of three independent experiments. ** P < 0.01, *** P < 0.001, **** P < 0.0001. B The image shows the exposure of finished reaction tubes (Ov-RPA–CRISPR/Cas12a) to UV light. Tubes 1–8: NTC, O. viverrini DNA at concentrations of 10, 1, 10−1, 10−2, 10−3, 10−4, and 10−5 ng after dilution with Milli-Q sterile water. All reaction tubes were visually inspected using an UV transilluminator and photographed using a phone camera. C The image shows agarose gel electrophoresis results of purified RPA products obtained from finished reactions. Lane M: 1 kb ladder. Lanes 1–8: NTC, O. viverrini DNA at concentrations of 10, 1, 10−1, 10−2, 10−3, 10−4, and 10−5 ng. The target amplicons (281 bp) were amplified at O. viverrini DNA concentrations of 10, 1, and 10−1 ng. One of the three replicates was subjected to gel electrophoresis and UV light assessment. D Fluorescence values of NTC, NC (O. viverrini-negative human copro-DNA), and O. viverrini DNA at concentrations of 10, 1, 10−1, 10−2, 10−3, 10−4, and 10−5 ng spiked in 100 ng of NC. E Tubes 1–9: NTC, NC, O. viverrini DNA at concentrations of 10, 1, 10−1, 10−2, 10−3, 10−4, and 10−5 ng spiked in 100 ng of NC. F Lane M: 1 kb ladder. Lanes 1–9: NTC, NC, O. viverrini DNA at concentrations of 10, 1, 10−1, 10−2, 10−3, 10−4, and 10−5 ng. Lane 10: 10−1 ng of O. viverrini DNA (spiked in Milli-Q sterile water). The target amplicons (281 bp) were amplified at O. viverrini DNA concentrations of 10, 1, and 10−1 ng (red arrowhead)