Fig. 3From: Stable expression of mucin glycoproteins GP40 and GP15 of Cryptosporidium parvum in Toxoplasma gondiiVerification of the expression of the GP40 and GP15ΔGPI of Cryptosporidium parvum in Toxoplasma gondii by immunofluorescence assay. a CpGP40 and CpGP15ΔGPI expressed in T. gondii. T. gondii RHΔku80 tachyzoites were transfected with pGRA1-CpGP40 or pGRA1-CpGP15Δgpi and allowed to infect HFF monolayers. IFA was performed 24 h after infection with anti-GP60 and -HA followed by Alexa Fluor-488-conjugated and 594-conjugated secondary antibodies. The far left panel shows the DIC field, and the far right panel shows a merged fluorescence image of the same field. DAPI-stained nuclei are in blue, Alexa Fluor-488 is in green, and Alexa Fluor-594 is in red. b CpGP40 expressed in T. gondii. IFA was performed 24 h after infection with anti-GP60 and -Strep followed by Alexa Fluor-488-conjugated and 594-conjugated secondary antibodies. c High-power images of the IFA to show the fine localization of the GP40 expression. CpGP40 proteins expressed in T. gondii are localized to the parasitophorous vacuole. Scale bars = 5 μmBack to article page