Table 1 Gene identification, primer sets of Babesia bovis genes of interest used for quantitative RT-PCR
From: Expression of sex-specific molecular markers by Babesia bovis gametes
B. bovis gene | Locus tag | Forward primers (5′-3′) | Reverse primers (5′-3′) |
|---|---|---|---|
cAMPDPK (PKA) | BBOV_I004190 | GACTTTGGATTTGCCAAGGA | GGAAGCATGAAATGGAGGAA |
hap2 | BBOV_III006770 | AAAGCGTCTATGTAATCAA | ACAGTTTTCTTCTCGTCA |
α-tubulin II | BBOV_III002820 | CATGCTTGACAACGAGGCTA | TGCGAGGGTAAGGTACCAAG |
znfp2 | BBOV_III011400 | GATTTCGCTCACGGAAACAT | TTGATGCCACATCATTGCTT |
α-tubulin I | BBOV_IV004290 | AGGGGTGCTTACAAGGGACT | GCCGAAAAGAAAAGGAAACC |
ABC transporter | BBOV_III011170 | AGTGGTTCCTGTCAGCCAAG | AGCTTCCAGTAGCGTGGGTA |
trap1 | BBOV_II002650 | ACCACTTACTCA ACTCCAACT | GTACCGGCCATCCAATCA A |
trap2 | BBOV_II002890 | GTCATGAGTATTCCCAGCCTT C | TCACTTCCTTCCGATGCTTTC |
trap3 | BBOV_II002630 | AACCTACCCAAACCGGAA AT | AGTCGTTGTTACTTGTCTCCT C |
trap4 | BBOV_II002870 | TTTGGATACCGCTGTGCTAC | CGGCTAGTCAAGGTCGTTAAA |
ccp1 | BBOV_III006360 | TGTATGGTATTCGTCAGTT | GTCGTCTATCACTTCACC |
ccp2 | BBOV_II003700 | TTAGCCGTTGATAGACTT | CTGCTGTGGTTTGTAATAG |
ccp3 | BBOV_III008930 | CTTCATTACACCACTCCT | AAGACACCATCAAACATAG |
Actin | BBOV_IV009790 | GAACGCCTGTCATTCGAGTT | GAAGCAAGCACCTTTCCAAC |