Fig. 2

Anti-proliferative and anti-invasion effect of BAM15 on Toxoplasma gondii. A Anti-proliferative effect of BAM15 on T. gondii-infected Vero cells was detected by qPCR. The inhibition rate of tachyzoite proliferation in each group was compared with that in the control group. B Dose-response curve of BAM15 against T. gondii proliferation. Data are presented as the mean ± SD of three independent experiments. *p < 0.01 compared with the control group. C Anti-invasion rate of BAM15 in T. gondii-infected Vero cells. D Dose-response curve of BAM15 against T. gondii invasion. Three independent experiments were performed, and the data are expressed as the mean ± SD. *p < 0.01 compared with the control group. E Plaques formed by T. gondii-infected cells after BAM15 treatment. BAM15 (12.5, 2.5, 0.5, 0.1, 0.02 or 0 μM) decreased T. gondii growth inhibition in Vero cells. F, G Anti-invasion effect of BAM15 was observed by fluorescence microscope. T. gondii RH-GFP strain was treated with BAM15 (1.25 or 12.5 μM), pyrimethamine or without drug as a control for 2 h before infecting Vero cells. Scale bars: 50 μm