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Table 1 Influence of time of experiment on mortality at 24 h

From: Time of exposure and assessment influence the mortality induced by insecticides against metabolic resistant mosquitoes

Experiment period

Resistant profile

Insecticides and synergist

Time of assay

Total

Dead

% Mortality at 24 h

(95% CI)

ORa (95% CI)

P-value

First

Metabolic resistant (Anopheles arabiensis and Culex quinquefasciatus)

DM

Day

300

141

47.0 (36.1–57.9)

1.00

 

Evening

300

103

34.3 (23.5–45.1)

0.14 (0.06–0.30)

 < 0.0001

DM + PBO

Day

300

131

43.7 (34.2–53.2)

1.00

 

Evening

300

90

30.0 (20.9–39.1)

0.29 (0.18–0.49)

 < 0.0001

Fully susceptible (Aedes aegypti)

DM

Day

120

120

100

1.00

 

Evening

120

120

100

1.00

–

DM + PBO

Day

120

94

78.3 (70.5–86.2)

1.00

 

Evening

120

86

71.7 (61.9–81.4)

0.70 (0.39–1.26)

0.234

Second

Metabolic resistant (Anopheles arabiensis, An. funestus and Cx. quinquefasciatus)

DM

Afternoon

360

249

69.2 (59.3–79.0)

1.00

 

Night

360

234

65.0 (54.9–75.1)

0.40 (0.19–0.82)

0.013

DM + PBO

Afternoon

360

276

76.7 (68.3–85.0)

1.00

 

Night

360

273

75.8 (67.4–84.3)

0.89 (0.51–1.55)

0.669

Knock

down resistant (An. gambiae)

DM

Afternoon

120

114

95.0 (90.1–99.9)

1.00

 

Night

120

119

99.2 (97.5–100)

6.26 (0.74–52.84)

0.092

DM + PBO

Afternoon

120

120

100

1.00

 

Night

120

120

100

1.00

–

Fully Susceptible (An. gambiae and Aedes aegypti)

DM

Afternoon

240

240

100

1.00

 

Night

240

240

100

1.00

–

DM + PBO

Afternoon

240

240

100

1.00

 

Night

240

240

100

1.00

–

  1. Mortality in the negative control was  0% at 24 h
  2. Results are from a fixed effect binomial logistic regression
  3. Day or afternoon time is set as the reference, and mosquito strains are set as fixed effects
  4. a Adjusted Odds ratio
  5. First experiment tested ITENs and ITWS fabric and second experiment tested ITNs fabric and analysed separately