Table 2 Influence of longer holding time on mortality of laboratory-reared mosquitoes
Experiment period | Resistant profile | Insecticides and synergist | Hour of assessment | Total | Dead | % Mortality (95% CI) | ORa (95% CI) | P-value |
|---|---|---|---|---|---|---|---|---|
First | Metabolic resistant (Anopheles arabiensis and Culex quinquefasciatus) | DM | 24 | 600 | 244 | 40.7 (33.0–48.4) | 1.00 |  |
72 | 600 | 282 | 47.0 (39.8–54.2) | 1.44 (1.09–1.88) | 0.009 | |||
DM + PBO | 24 | 600 | 221 | 36.8 (30.2–43.5) | 1.00 |  | ||
72 | 600 | 297 | 49.5 (43.5–55.5) | 1.82 (1.42 –2.34) |  < 0.0001 | |||
Fully susceptible (Aedes aegypti) | DM | 24 | 240 | 240 | 100 | 1.00 | Â | |
72 | 240 | 240 | 100 | 1.00 | Â | |||
DM + PBO | 24 | 240 | 180 | 75.0 (68.8–81.2) | 1.00 |  | ||
72 | 240 | 169 | 70.4 (64.2–76.6) | 0.87 (0.64–1.19) | 0.381 | |||
Second | Metabolic resistant (Anopheles arabiensis, An. funestus and Cx. quinquefasciatus) | DM | 24 | 720 | 483 | 67.1 (60.1–74.1) | 1.00 |  |
72 | 720 | 496 | 68.9 (61.8–76.0) | 1.10 (0.87–1.39) | 0.410 | |||
DM + PBO | 24 | 720 | 549 | 76.3 (70.3–82.2) | 1.00 |  | ||
72 | 720 | 572 | 79.4 (74.1–84.8) | 1.14 (0.93–1.41) | 0.205 | |||
Knock Down resistant (Anopheles gambiae) | DM | 24 | 240 | 233 | 97.1 (94.5–99.7) | 1.00 |  | |
72 | 240 | 237 | 98.8 (96.9–100) | 1.07 (0.75–1. 52) | 0.720 | |||
DM + PBO | 24 | 240 | 240 | 100 | 1.00 |  | ||
72 | 240 | 240 | 100 | 1.00 | Â | |||
Fully susceptible (An. gambiae and Ae. aegypti) | DM | 24 | 480 | 480 | 100 | 1.00 | – | |
72 | 480 | 480 | 100 | 1.01 (0.78–1.30) | 0.948 | |||
DM + PBO | 24 | 480 | 480 | 100 | 1.00 | – | ||
72 | 480 | 480 | 100 | 1.02 (0.79–1.31) | 0.897 |