Fig. 1

Toxoplasma gondii sortilin was localized in the Golgi apparatus. A After permeabilization with 0.25% Triton X-100, the sortilin fluorescence signal (green) was localized near the nucleus (blue) of both extracellular and intracellular T. gondii. Without permeabilization, the sortilin fluorescence signal was not observed. B The fluorescence signal of sortilin (green) overlaps with that of the Golgi apparatus marker GROASP (red) in both intracellular and extracellular parasites. C The RH strain of T. gondii and 5 μg/ml BFA were co-cultured at 37 °C for 4 h (intracellular and extracellular). The fluorescence signals of sortilin (green) and GROASP (red) in both extracellular and intracellular parasites completely disappeared after BFA treatment