Fig. 2
From: Toxoplasma sortilin interacts with secretory proteins and it is critical for parasite proliferation
Immunoprecipitation validation of sortilin-interacting proteins. A Toxoplasma gondii proteins pulled down by the sortilin-specific antibody. B–E Confirmation of TgROP9, TgMIC3, and TgSAG2 co-precipitation with sortilin by respective protein-specific antibodies in Western blotting. Immunoprecipitates were detected with anti-TgROP9, anti-TgMIC3, and anti-TgSAG2 antibodies. Toxoplasma gondii total proteins were used as a positive control. Vero cell total proteins were used as a negative control. F Co-localization of TgROP9, TgMIC3, and TgSAG2 (green) with sortilin (red) in T. gondii