Fig. 1

Workflow for in silico scanning of proteins involved in extracellular vesicle biogenesis. Protein sequences from Homo sapiens involved in EV biogenesis were collected from the UniProt database and used to interrogate Sparicotyle chrysophrii genome and transcriptome datasets, simultaneously using tBLASTn. Functional domains of H. sapiens and S. chrysophrii hypothetical orthologues were compared using InterPro, Pfam domains were retrieved, and PsiPred was used to compare their secondary structures. The chosen S. chrysophrii sequences were then used in a BLASTx search against the NCBI database. Orthologue protein sequences from Neodermata were used to interrogate S. chrysophrii draft genome by tBLASTn and the same methodology was applied to identify hypothetical proteins