Occurrence and genetic variability of Phlebotomus papatasi in an urban area of southern Italy
© Dantas-Torres et al; licensee BioMed Central Ltd. 2010
Received: 26 July 2010
Accepted: 25 August 2010
Published: 25 August 2010
A phlebotomine sand fly was noticed in the second floor of an old building in a highly urbanized area of southern Italy. A short-term entomological survey was carried out in the subsequent weeks to this event, allowing the collection of additional phlebotomine sand flies that were later identified as Phlebotomus papatasi. We assessed the genetic variability among P. papatasi sequences obtained in this study and those available from Italy using a mitochondrial DNA (mtDNA) fragment (from cytochrome b gene to NADH1) and the internal transcribed spacer 2 (ITS2) as genetic markers.
From 9 June to 19 July, eight males and seven females (two blood-fed) of P. papatasi were collected in the old town of Bari (southern Italy). The insects were found near the bed and in the bathroom and potential blood sources (e.g., pigeons and dogs) for them were common in the neighbourhood. Again, five females of P. papatasi collected in Valenzano, another urban area in the province of Bari, were also identified and included in the genetic study. The mtDNA sequences (945 bp) obtained from Bari and Valenzano were identical except for a single transition (T ↔ C) at the 793 nucleotide residue. Pairwise comparison of the last 440 bp of the mtDNA fragment analyzed herein with other sequences of P. papatasi from Italy revealed a nucleotide variation ranging from 0.2 to 1.3%. Three ITS2 sequence types were detected within specimens collected in Valenzano, one of them identical to that from Bari. Pairwise comparison of ITS2 sequences of P. papatasi from Italy revealed a nucleotide variation up to 1.8%.
This study reports the occurrence of P. papatasi in an urban area of southern Italy and shows a low nucleotide difference among ITS2 and mtDNA sequences of this species available from Italy. The presence of P. papatasi in urban areas might represent a risk for human health, particularly for the potential transmission of sandfly fever viruses.
Phlebotomus papatasi is the type-species of the genus Phlebotomus, which include all phlebotomine sand fly vectors of Leishmania parasites in the Old World . The biology of P. papatasi has been subject of many field and laboratory investigations [2–8]. Through its range, P. papatasi is predominantly a domestic species, being often found in or nearby human habitations . This sand fly feeds readily on birds, rodents, domestic animals as well as on humans [10–12]. Moreover, P. papatasi is a vector of Leishmania major, the agent of zoonotic cutaneous leishmaniasis in North Africa and Middle East , and has been implicated in the transmission of viruses in Europe . Sandfly fevers were firstly described in southern Italy during the World War II, when an outbreak of influenza-like illness was recorded among US soldiers due to Sicilian and Naples viruses , which have been later isolated from P. papatasi in many Mediterranean countries [16, 17]. The reduced abundance of P. papatasi due to the control campaigns against malaria in the past century diminished the perceived relevance of this sand fly in Italy. However, the abundance of P. papatasi is increasing  and the actual risk for public health in Italy is probably underestimated. Indeed, surveys carried out in Italy before 1975 indicated that P. papatasi was already widespread, thought rarely abundant . However, studies conducted in central and southern Italy from 1975 onwards indicated an increase in abundance of this sand fly, especially in urban areas . In spite of its widespread distribution, data on the public health significance of P. papatasi in Italy is scant and little is known about the genetic relationships among geographically isolated populations.
On 8 June 2009, a phlebotomine sand fly was noticed inside the apartment of one of us (FTD). This episode occurred on the second floor of an old, restructured building located downtown, in a highly urbanized area of southern Italy. Herein, further worth noting facts of this history are presented along with genetic data of this P. papatasi population, which is one of the southernmost records of this species in Europe. The possible public health implications of the presence of P. papatasi in urban areas of Italy are discussed.
One phlebotomine sand fly from Bari was processed for DNA extraction and polymerase chain reaction (PCR) amplification of fragments of the mitochondrial - from cytochrome b (cytb) to NADH dehydrogenase subunit 1 (NADH1) - and of the ribosomal DNA - internal transcribed spacer 2 (ITS2) - as previously described [19–21]. Additionally, five specimens of P. papatasi collected from another urban area from the province of Bari (Valenzano, 41°3'N, 16°53'E) were processed for comparison purposes. In brief, DNA was extracted using DNeasy Blood & Tissue Kit (Qiagen), according to the manufacturer's instructions. The PCR was carried out in a 50 μl final volume, which included 4 μl of DNA template, 10 mM Tris-HCl (pH 8.3), 50 mM KCl, 2.5 mM MgCl2, 200 μM of each dNTP, 50 pmol of each primer and 1.25 U of AmpliTaq Gold (Applied Biosystems). Negative controls (no DNA) were included in each PCR run and amplicons were visualised by 2% agarose gel electrophoresis under ultraviolet exposure. PCR products were purified using Ultrafree-DA columns (Amicon; Millipore) and sequenced with an automated sequencer (ABI-PRISM 377; Applied Biosystems). DNA sequences were determined on both strands and deposited in the GenBank database (GenBank:HM992926, GenBank:HM992927, GenBank:HM992928, GenBank:HM992929, GenBank:HM992930). The electropherogram of each sequence was verified by eye, and the sequences were aligned manually using ClustalX program . Sequences were compared by BLAST analysis with cytb and ITS2 sequences of P. papatasi available in the GenBank database. Pairwise comparison between sequences from Italy obtained in this study and those available in GenBank was carried out and nucleotide differences (D) were calculated .
The mtDNA sequences (945 bp) obtained from Bari and Valenzano were identical except for a single transition (T ↔ C) at the 793 nucleotide residue. Moreover, BLAST analysis with the longest consensus cytb sequence (717 bp) available in Genbank (provenience Tunisia; GenBank:AF161214), revealed a high homology (99.9%) with the sequence from Bari. Pairwise comparison of the last 440 bp of the mtDNA fragment analyzed herein with those of P. papatasi from Lazio (Italy) available in GenBank (GenBank:DQ381829, GenBank:DQ381830, GenBank:DQ381831) revealed a nucleotide variation ranging from 0.2 to 1.3%.
Three ITS2 sequence types were detected within specimens collected in Valenzano (i.e., nos. 1, 2 and 3), differing only by some insertions/deletions. The sequence type no. 1 was identical to that from Bari. In particular, ITS2 sequences obtained had an overall length ranging from 483 to 490 bp and presented high homologies (99-100%) with those from Syria (GenBank: DQ887666) and Cyprus (GenBank: DQ887634), respectively. Pairwise comparison of ITS2 sequences of P. papatasi from Italy available in GenBank (GenBank:EF408790, GenBank:EF408791) revealed a nucleotide difference up to 1.8%.
In this study, a population of P. papatasi was detected in an urban area of southern Italy, representing one of the southernmost records of this species in Europe. The morphology of both males and females of P. papatasi examined in the present study perfectly fits with the species description provided by Lewis . Genetically, the specimens sequenced in this study are closely related with P. papatasi from Tunisia (cytb), Cyprus and Syria (ITS2). In particular, the nucleotide differences within the mtDNA sequences from Apulia herein characterized increased when compared with those from Lazio. Nonetheless, the sequence differences found in this study are consistent with those found in other studies using the same genetic markers [24, 25]. These DNA fragments, particularly cytb, have been regarded as good genetic markers to assess the relationships between closely related populations of P. papatasi in the Mediterranean region . Accordingly, our results indicate a low level of genetic variability among Italian populations of P. papatasi, which is in line with a recent phylogenetic study reporting a genetic homogeneity among 26 populations of P. papatasi from 18 countries .
Whether the presence of P. papatasi inside houses in urban areas in Italy or other southern European countries represents a risk for human health, particularly for their potential role as vectors of phleboviruses and Leishmania parasites, remains uncertain. Again, the natural vertebrate hosts of L. major include some wild rodents  whose absence in Europe might represent one of the reasons for the absence of this zoonotic agent in southern Europe . Indeed, it has been stated that the risk of emergence of cutaneous leishmaniasis by L. major in Europe is low because its main gerbil reservoirs are absent, even though P. papatasi is locally abundant in some areas . However, a recent study suggested that Norwegian rats (Rattus norvegicus), which are abundant in Europe, could be involved in the maintenance of L. major in southern Iran . Indeed, the risk of introduction of L. major in southern Europe has not been fully evaluated and deserves more attention.
This work provides genetic data of a population of P. papatasi in an urban area of southern Italy, representing one of the southernmost reports of this sand fly in Europe. Finally, further research to assess whether the presence of P. papatasi in urban areas of southern Europe represents a risk for public health is needed.
- Lewis DJ: A taxonomic review of the genus Phlebotomus (Diptera: Psychodidae). Bull Br Museum Entomol. 1982, 45: 121-209.Google Scholar
- Valevich TA, Dergacheva TI: [The laboratory cultivation of Phlebotomus papatasi. The nature of the reaction of the sandflies to unfavorable environmental conditions]. Med Parazitol (Mosk). 1992, 1: 18-21.Google Scholar
- Srinivasan R, Panicker KN: Laboratory observations on the biology of the phlebotomid sandfly, Phlebotomus papatasi (Scopoli, 1786). Southeast Asian J Trop Med Public Health. 1993, 24: 536-539.PubMedGoogle Scholar
- Kasap OE, Alten B: Laboratory estimation of degree-day developmental requirements of Phlebotomus papatasi (Diptera: Psychodidae). J Vector Ecol. 2005, 30: 328-333.PubMedGoogle Scholar
- Kasap OE, Alten B: Comparative demography of the sand fly Phlebotomus papatasi (Diptera: Psychodidae) at constant temperatures. J Vector Ecol. 2006, 31: 378-385. 10.3376/1081-1710(2006)31[378:CDOTSF]2.0.CO;2.View ArticlePubMedGoogle Scholar
- Abdel-Hamid RM: The effect of the host blood on the biology of the sand fly, Phlebotomus papatasi (Diptera: Psychodidae) under laboratory conditions. J Egypt Soc Parasitol. 2007, 37: 1091-1100.PubMedGoogle Scholar
- Benkova I, Volf P: Effect of temperature on metabolism of Phlebotomus papatasi (Diptera: Psychodidae). J Med Entomol. 2007, 44: 150-154. 10.1603/0022-2585(2007)44[150:EOTOMO]2.0.CO;2.View ArticlePubMedGoogle Scholar
- Chelbi I, Zhioua E: Biology of Phlebotomus papatasi (Diptera: Psychodidae) in the laboratory. J Med Entomol. 2007, 44: 597-600. 10.1603/0022-2585(2007)44[597:BOPPDP]2.0.CO;2.View ArticlePubMedGoogle Scholar
- Feliciangeli MD: Natural breeding places of phlebotomine sandflies. Med Vet Entomol. 2004, 18: 71-80. 10.1111/j.0269-283X.2004.0487.x.View ArticlePubMedGoogle Scholar
- Bongiorno G, Habluetzel A, Khoury C, Maroli M: Host preferences of phlebotomine sand flies at a hypoendemic focus of canine leishmaniasis in central Italy. Acta Trop. 2003, 88: 109-116. 10.1016/S0001-706X(03)00190-6.View ArticlePubMedGoogle Scholar
- Palit A, Bhattacharya SK, Kundu SN: Host preference of Phlebotomus argentipes and Phlebotomus papatasi in different biotopes of West Bengal, India. Int J Environ Health Res. 2005, 15: 449-454. 10.1080/09603120500392525.View ArticlePubMedGoogle Scholar
- Velo E, Paparisto A, Bongiorno G, Di Muccio T, Khoury C, Bino S, Gramiccia M, Gradoni L, Maroli M: Entomological and parasitological study on phlebotomine sandflies in central and northern Albania. Parasite. 2005, 12: 45-49.View ArticlePubMedGoogle Scholar
- Lane RP: Sand flies (Phlebotominae). Medical Insects and Arachnids. Edited by: Lane RP, Crosskey RW. 1993, London: Chapman and Hall, 78-119.View ArticleGoogle Scholar
- Depaquit J, Grandadam M, Fouque F, Andry PE, Peyrefitte C: Arthropod-borne viruses transmitted by Phlebotomine sandflies in Europe: a review. Euro Surveill. 2010, 15: 19507-PubMedGoogle Scholar
- Sabin AB, Philip CB, Paul JR: Phlebotomus (pappataci or sandfly) fever. A disease of military importance, summary of existing knowledge and preliminary report of original investigations. JAMA. 1944, 125: 603-606.View ArticleGoogle Scholar
- Nicoletti L, Ciufolini MG, Fortuna C, Magurano F, Fiorentini C, Marchi A, Benedetti E, Bucci P: Arboviruses in Italy. Parassitologia. 2008, 50: 109-111.PubMedGoogle Scholar
- Brett-Major DM, Claborn DM: Sand fly fever: what have we learned in one hundred years?. Mil Med. 2009, 174: 426-431.View ArticlePubMedGoogle Scholar
- Maroli MN, Bettini S: Past and present prevalence of Phlebotomus papatasi (Diptera: Psychodidae) in Italy. Parasite. 1997, 4: 273-276.View ArticleGoogle Scholar
- Simon C, Frati F, Beckenbach A, Crespi B, Liu H, Flook P: Evolution, weighting, and phylogenetic utility of mitochondrial gene sequences and a compilation of conserved polymerase chain reaction primers. Ann Entomol Soc Am. 1994, 87: 651-701.View ArticleGoogle Scholar
- Esseghir S, Ready PD, Killick-Kendrick R, Ben-Ismail R: Mitochondrial haplotypes and phylogeography of Phlebotomus vectors of Leishmania major. Insect Mol Biol. 1997, 6: 211-225. 10.1046/j.1365-2583.1997.00175.x.View ArticlePubMedGoogle Scholar
- Depaquit J, Ferté H, Léger N, Killick-Kendrick R, Rioux JA, Killick-Kendrick M, Hanafi HA, Gobert S: Molecular systematics of the phlebotomine sandflies of the subgenus Paraphlebotomus (Diptera, Psychodidae, Phlebotomus) based on ITS2 rDNA sequences. Hypotheses Of dispersion and speciation. Insect Mol Biol. 2000, 9: 293-300. 10.1046/j.1365-2583.2000.00179.x.View ArticlePubMedGoogle Scholar
- Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG: The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 1997, 25: 4876-4882. 10.1093/nar/25.24.4876.PubMed CentralView ArticlePubMedGoogle Scholar
- Chilton NB, Gasser RB, Beveridge I: Differences in a ribosomal DNA sequence of morphologically indistinguishable species within the Hypodontus macropi complex (Nematoda: Strongyloidea). Int J Parasitol. 1995, 25: 647-651. 10.1016/0020-7519(94)00171-J.View ArticlePubMedGoogle Scholar
- Hamarsheh O, Presber W, Abdeen Z, Sawalha S, Al-Lahem A, Schoenian G: Genetic structure of Mediterranean populations of the sandfly Phlebotomus papatasi by mitochondrial cytochrome b haplotype analysis. Med Vet Entomol. 2007, 21: 270-277. 10.1111/j.1365-2915.2007.00695.x.View ArticlePubMedGoogle Scholar
- Depaquit J, Lienard E, Verzeaux-Griffon A, Ferté H, Bounamous A, Gantier JC, Hanafi HA, Jacobson RL, Maroli M, Moin-Vaziri V, Müller F, Ozbel Y, Svobodova M, Volf P, Léger N: Molecular homogeneity in diverse geographical populations of Phlebotomus papatasi (Diptera, Psychodidae) inferred from ND4 mtDNA and ITS2 rDNA Epidemiological consequences. Infect Genet Evol. 2008, 8: 159-170. 10.1016/j.meegid.2007.12.001.View ArticlePubMedGoogle Scholar
- Hamarsheh O, Presber W, Al-Jawabreh A, Abdeen Z, Amro A, Schönian G: Molecular markers for Phlebotomus papatasi (Diptera: Psychodidae) and their usefulness for population genetic analysis. Trans R Soc Trop Med Hyg. 2009, 103: 1085-1086. 10.1016/j.trstmh.2009.02.011.View ArticlePubMedGoogle Scholar
- Ashford RW: Leishmaniasis reservoirs and their significance in control. Clin Dermatol. 1996, 14: 523-532. 10.1016/0738-081X(96)00041-7.View ArticlePubMedGoogle Scholar
- Ready PD: Leishmaniasis emergence in Europe. Euro Surveill. 2010, 15: 19505-PubMedGoogle Scholar
- Motazedian MH, Parhizkari M, Mehrabani D, Hatam G, Asgari Q: First Detection of Leishmania major in Rattus norvegicus from Fars Province, Southern Iran. Vector Borne Zoonotic Dis.
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