Polymorphic microsatellites in the human bloodfluke, Schistosoma japonicum, identified using a genomic resource

Xiao, Ning; Remais, Justin; Brindley, Paul J; Qiu, Dongchuan; Spear, Robert; Lei, Yang; Blair, David

doi:10.1186/1756-3305-4-13

Parasites & Vectors

Table 1 Primer sequences and other characteristics for each of 22 microsatellite loci amplified from 20 specimens of Schistosoma japonicum

From: Polymorphic microsatellites in the human bloodfluke, Schistosoma japonicum, identified using a genomic resource

Locus name	Primer sequences (tails removed)	Repeat unit	Length of locus in Sj Genome draft (excl tails)	Length range (PCR product - bp excl tail)	No. of alleles	In HWE?	Ho	He
SjP4	F:ACAAGCTCCAATCGTCTCTGA R:GAATACTGCCGCCCTTGTAA	TAA	217	182-244	14		0.789	0.831
SjP9	F:GATGAAACAGATACCCAGCAC R:TGCATGTAAAAATGGCTTGC	TAA	283	239-301	14	**	0.600	0.915
SjP18	F:TCCTTTATCTGGGCTGTGGA R:TTTCAGCAGGATAACATGACG	TGA	286	261-298	7		0.684	0.703
SjP19	F:GGTATCTTCGCTTTTTAGCATGG R:TCCTAGGGTGTGGTATCAGAG	ATT	196	161-257	12	**	0.737	0.896
SjP22	F:CAAAGCCTAAACGTCATAGACAG R:CAACCACCGATAAGTAGAGTGGA	TTA	150	105-167	11	*	0.850	0.892
SjP23	F:GTACGATATGAGGGAAAGTTCA R:CTCTCCTTCAGACGAATTGAG	TAA	219	192-253	14		1.00	0.933
SjP26	F:CAAGGGAACATTGTACATGAAG R:TGGTAAAGGAGAAAGTGAACG	TAA	307	229-312	7		0.700	0.659
SjP28	F:TAACGCCTTTTCCCACATTC R:ATAACCACGATGGGAACCAA	TTA	242	232-269	13	**	0.900	0.926
SjP32	F:TGTCACCGAGTCTTCATTAGC R:ACAGTCAGTAGACCTGGATAAAC	TTA	175	142-192	14		0.950	0.929
SjP34	F:GGCGACCATACATAAGGAGAAT R:GACCGATTTCTAATGGAGCA	TAA	409	381-430	13	*	0.778	0.908
SjP37	F:TCCTTGACACGAGGTACATGT R:ATTACGTAACAGAAGGCTGGA	TAT	290	240-314	8		0.833	0.714
SjP39	F:GACGACTGTTAAGTCCATCTGA R:ATAACCAATCTCCACGAAAGC	ATT	239	223-251	8		0.900	0.871
SjP42	F:GCTGCAGCTTCTGTGTAGTAA R:GTCTTGCTCAGATCAGTTCGT	TAA	216	199-234	9		0.950	0.855
SjP43	F:ACAATGGCTATTGGTTGAGTAG R:GGAGCATGCGTATATGGAAAA	TAA	188	184-235	9		0.750	0.854
SjP45	F:ATAACACCGAATCTGTTCAGC R:TAATCCGGTCAGGATGTATGT	TAT	156	150-244	13		0.850	0.921
SjP48	F:TTGTTGGGTAGTGATGGTAGG R:TAGTTCATTCCACCTCTTGGA	TAA	246	251-273	6		0.650	0.795
SjP54	F:TTAGGCTTGTTGGTGCTGATA R:AGGTAAAGCAAATCCCATAGC	GGTA	437	373-446	9		1.00	0.712
SjP58	F:TCCCAGTACCAATGTAGATGTG R:CTAATAAAGTCGTCAAGGAGCA	AAT	227	439-499	12	**	0.800	0.921
SjP60	F:CGATTCATTCATAGCCTGACT R:GAATCCCATCACAGATTAACG	TAT	155	134-165	10		0.900	0.867
SjP61	F:TCATCTTGTCACCAACTAGGC R:GCTTGGAGGAGGCTAAAATAC	TAT	188	158-238	9		0.700	0.679
SjP63	F:ACCGCCACTACCACTAACCTCA R:TTGACCTGAAATCTGTCCCTA	TAA	390	333-389	13	**	0.800	0.904
SjP88	F:GCTTTCCAGGCATAAACTTCAC R:TCTCCTAATGATGGGAACAG	TAA	408	380-417	9		0.950	0.887

Sequences of microsatellites reported here have been deposited in GenBank [AB604199 - AB604218].
Note that the first two loci are from [10] and are included for comparative purposes.
The annealing temperature of PCRs for all loci is 55°C
* P < 0.05 ** P < 0.01 as determined in GenAlEx.

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ISSN: 1756-3305

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