Notes on the blood-feeding behavior of Aedes albopictus (Diptera: Culicidae) in Cameroon
© Kamgang et al; licensee BioMed Central Ltd. 2012
Received: 12 January 2012
Accepted: 21 March 2012
Published: 21 March 2012
The invasive mosquito Aedes albopictus is often considered a poor vector of human pathogens, owing to its catholic feeding behavior. However, it was recently incriminated as a major vector in several Chikungunya epidemics, outside of its native range. Here we assessed two key elements of feeding behavior by Ae. albopictus females in Yaoundé, Cameroon, Central Africa. Host preference was explored and the human-biting activity of females was monitored over 24 h to determine periods of maximum bite exposure.
Analysis of ingested blood in outdoor-resting females showed that Ae. albopictus preferentially fed on humans rather than on available domestic animals (95% of the blood meals contained human blood). Our results further showed that Ae. albopictus is a day-biting species in Yaoundé, with a main peak of activity in the late afternoon.
This is the first report on the feeding behavior of Ae. albopictus in Central Africa. The species is highly aggressive to humans and might therefore be involved in human-human virus transmission in this setting.
KeywordsAedes albopictus Feeding behavior Host preference Nychthemeral activity Cameroon
Aedes albopictus (Skuse, 1894) is an invasive mosquito species that originated in Asian forests  and expanded to temperate and tropical regions of America, Africa and Europe, mainly during the last three decades . Aedes albopictus is widely considered as a secondary vector of human arboviruses such as dengue virus (DENV), because it is thought to preferentially feed on animals rather than humans, contrary to the highly anthropophilic Aedes aegypti (L., 1762) . Yet, Ae. albopictus has been incriminated as a primary vector in recent chikungunya virus (CHIKV) epidemics in the Indian Ocean, Central Africa and Europe [4–6], suggesting sustained man-vector contact in these settings. In Cameroon (Central Africa), Ae. albopictus was first recorded in the early 2000s  and rapidly spread throughout the south of the country . In Cameroonian urban centers such as Yaoundé, the species now pullulates  and is gradually replacing autochthonous Ae. aegypti populations. Concomitant with the spread of Ae. albopictus in Central Africa, an increase in DENV and CHIKV outbreaks was reported in a number of countries, including Cameroon , Gabon [5, 11] and the Republic of Congo . Indeed, Cameroonian Ae. albopictus populations were shown to be orally susceptible to DEN-2 virus and CHIKV infection , and the species was recognized as the main vector of both viruses in 2007 in Libreville, Gabon [5, 11].
Although Ae. albopictus preferentially bites mammals , females can also feed on most groups of vertebrates, both cold- and warm-blooded, including reptiles, birds and amphibians . Such feeding plasticity, which has been found to vary according to the geographic origin of mosquito populations , maximizes the fitness (fecundity and survival) of Ae. albopictus and enhances the risk that it may propagate zoonotic pathogens from wildlife or domestic animals to humans . In addition to host preferences, the intensity of human-vector contact and, therefore, the risk of pathogen transmission, is modulated by the biting period(s) along the nychthemere, which impacts on host availability. Although geographic variations have been recorded in the biting rhythm of Ae. Albopictus females, the species usually bites outdoors and during daytime, with two main peaks of activity in the morning and early evening [3, 4, 14]. To our knowledge, there are no published data on the feeding behavior of Ae. albopictus in Central Africa. In order to fill this gap, we conducted a study of Ae. albopictus host preferences and biting activity in Yaoundé, Cameroon.
Mosquitoes were collected from two sites in the periphery of Yaoundé, the capital city of Cameroon: "Club France" (N3°50'28", E11°30'2") in the Efoulan district and "Club hippique" (N3°54'05", E11°29'53") in the Ntougou district. "Club France", a leisure center (tennis, swimming pool, banquets, etc.;), is surrounded by dense vegetation which provides potential resting and oviposition sites for Aedes mosquitoes. "Club hippique", an equestrian center, is also surrounded by dense vegetation. The horses (N > 25) provide potential blood sources for mosquitoes. Additionally, both sites are surrounded by human dwellings with domestic animals such as dogs, pigs and chickens.
Field collections were conducted from April to early July 2009, during the rainy season when mosquito densities are highest. To determine the feeding preferences of Ae. albopictus, wild blood-fed females were caught in both study sites. The low vegetation that constitutes resting sites was stirred up to disturb mosquitoes, and flying females were caught by sweep-netting. Each capture was done by a single collector and lasted 30 min. Collected mosquitoes were individually transferred to 10-mL glass vials using a mouth aspirator; they were identified morphologically by reference to standard keys  and stored at -20°C until blood meal analysis. The abdomen of freshly-fed Ae. albopictus and Ae. aegypti females was dissected out and used as a template for blood-meal analysis by ELISA , using anti-human, -horse, -bird, -reptile, -swine, -sheep and -dog antibodies (Sigma Aldrich, St. Louis, MO). To explore the biting activity of Ae. albopictus, blood-seeking females were trapped outdoors over entire 24-hour periods using a double-net device baited with a human volunteer, as described in Delatte et al. . The experiment was repeated eight times in "Club France" only, between April and July 2009.
Results and discussion
Ingested-blood analysis in Ae.albopictus females from sites in Yaoundé, Cameroon
Number of specimens analyzed (% positive)
This study, which provides the first data on the feeding behavior of Ae. albopictus in Central Africa, demonstrates that this species readily bites humans in urban settings and might therefore play a major role in human-human transmission of emerging arboviruses such as DENV and CHIKV. Detailed knowledge of the feeding behavior of this vector species, such as the rhythm of females' biting activity, is needed to develop and implement efficient personal protection tools against Ae. albopictus, especially in newly colonized areas.
We thank Rose Nyambam for technical assistance with ELISA analysis. This research was supported by the French government through "Agence Nationale pour la Recherche", that funded the Epidengue project (ANR 05 SEST 010-01). Basile Kamgang was supported by an IRD/DSF PhD fellowship.
- Smith CEG: The history of dengue in tropical Asia and its probable relationship to the mosquitoes Aedes aegypt. J Trop Med Hyg. 1956, 59: 3-11.Google Scholar
- Gratz NG: Critical review of the vector status of Aedes albopictu. Med Vet Entomol. 2004, 18: 215-227. 10.1111/j.0269-283X.2004.00513.x.View ArticlePubMedGoogle Scholar
- Hawley AH: The biology of Aedes albopictu. J Am Mosq Control Assoc. 1988, 4: 2e39-Google Scholar
- Paupy C, Delatte H, Bagny L, Corbel V, Fontenille D: Aedes albopictu, an arbovirus vector: from the darkness to the light. Microbes Infect. 2009, 11: 1177-85. 10.1016/j.micinf.2009.05.005.View ArticlePubMedGoogle Scholar
- Paupy C, Ollomo B, Kamgang B, Moutailler S, Rousset D, Demanou M, Hervé JP, Leroy E, Simard F: Role of Ae. albopictus versus Ae. aegypti in dengue and chikungunya emergences in Central Africa: laboratory and field evidence. Vector Borne and Zoonotic Dis. 2010, 10 (3): 259-66. 10.1089/vbz.2009.0005.View ArticleGoogle Scholar
- Pagès F, Peyrefitte CN, Mve MT, Jarjaval F, Brisse S, Iteman I, Gravier P, Tolou H, Nkoghe D, Grandadam M: Aedes albopictu mosquito: the main vector of the 2007 Chikungunya outbreak in Gabon. PLoS One. 2009, 4 (3): e4691-10.1371/journal.pone.0004691.PubMed CentralView ArticlePubMedGoogle Scholar
- Fontenille D, Toto JC: Aedes (Stegomyia) albopictu (Skuse), a potential new dengue vector in Southern Cameroon. Emerg Infect Dis. 2001, 7: 1066-1067. 10.3201/eid0706.010631.PubMed CentralView ArticlePubMedGoogle Scholar
- Simard F, Nchoutpouen E, Toto JC, Fontenille D: Geographic distribution and breeding site preference of Aedes albopictu and Aedes aegypt (Diptera: Culicidae) in Cameroon, Central Africa. J Med Entomol. 2005, 42: 726-731. 10.1603/0022-2585(2005)042[0726:GDABSP]2.0.CO;2.View ArticlePubMedGoogle Scholar
- Kamgang B, Happi JY, Boisier P, Njiokou F, Hervé JP, Simard F, Paupy C: Geographic and ecological distribution of the dengue and chikungunya virus vectors Aedes aegypt and Aedes albopictu in three major Cameroonian towns. Med Vet Entomol. 2010, 24 (2): 132-41. 10.1111/j.1365-2915.2010.00869.x.View ArticlePubMedGoogle Scholar
- Peyrefitte CN, Rousset D, Pastorino BA, Pouillot R, Bessaud M, Tock F, Mansaray H, Merle OL, Pascual AM, Paupy C, Vessiere A, Imbert P, Tchendjou P, Durand JP, Tolou HJ, Grandadam M: Chikungunya virus, Cameroon, 2006. Emerg Inf Dis. 2007, 13: 768-771.View ArticleGoogle Scholar
- Leroy EM, Nkoghe D, Ollomo B, Nze-Nkogue C, Becquart P, Grard G, Pourrut X, Charrel R, Moureau G, Ndjoyi-Mbiguino A, De-Lamballerie X: Concurrent chikungunya and dengue virus infections during simultaneous outbreaks, Gabon. Emerg Inf Dis. 2009, 15: 591-593. 10.3201/eid1504.080664.View ArticleGoogle Scholar
- Kelvin AA: Outbreak of Chikungunya in the Republic of Congo and the global picture. J Infect Dev Ctries. 2011, 5 (6): 441-444.View ArticlePubMedGoogle Scholar
- Scholte EJ, Schaffner F: Waiting the tiger: establishment and spread of the Aedes albopictus mosquito in Europe. Emerging Pests and Vector-Borne Diseases in Europe. Edited by: Takken W, Knols BGJ. 2007, Wageningen: Wageningen Academic Publishers, 241-260.Google Scholar
- Delatte H, Desvars A, Bouétard A, Bord S, Gimonneau G, Vourc'h G, Fontenille D: Blood-feeding behavior of Aedes albopictu, a vector of Chikungunya on La Réunion. Vector Borne Zoonotic Dis. 2010, 10 (3): 249-58. 10.1089/vbz.2009.0026.View ArticlePubMedGoogle Scholar
- Gubler DJ: Aedes albopictu in Africa. Lancet Infect Dis. 2003, 3: 751-752. 10.1016/S1473-3099(03)00826-0.View ArticlePubMedGoogle Scholar
- Jupp PG: Mosquitoes of Southern Africa: Culicinae and Toxorhynchitinae. 1996, Hartebeespoort, South Africa: Ekogilde Publishers, 156-Google Scholar
- Beier JC, Perkins PV, Wirtz RA, Koros J, Diggs D, Gargam-II TP, Koech DK: Bloodmeal identification by direct enzyme-linked immunosorbent assay (ELISA) tested on Anopheles (Diptera: Culicidae) in Kenya. J Med Entomol. 1988, 25: 9-16.View ArticlePubMedGoogle Scholar
- Ponlawat A, Harrington LC: Blood feeding patterns of Aedes aegypt and Aedes albopictu in Thailand. J Med Entomol. 2005, 42 (5): 844-9. 10.1603/0022-2585(2005)042[0844:BFPOAA]2.0.CO;2.View ArticlePubMedGoogle Scholar
- Richards SL, Ponnusamy LT, Unnasch TR, Hassan HK, Apperson CS: Host-Feeding patterns of Aedes albopictu (Diptera: Culicidae) in relation to availability of human and domestic animals in suburban landscapes of central North Carolina. J Med Entomol. 2006, 43 (3): 543-551. 10.1603/0022-2585(2006)43[543:HPOAAD]2.0.CO;2.PubMed CentralView ArticlePubMedGoogle Scholar
- Valerio L, Marini F, Bongiorno G, Facchinelli L, Pombi M, Caputo B, Maroli M, Della Torre A: Host-feeding patterns of Aedes albopictu (Diptera: Culicidae) in urban and rural contexts within Rome province, Italy. Vector Borne Zoonotic Dis. 2010, 10 (3): 291-4. 10.1089/vbz.2009.0007.View ArticlePubMedGoogle Scholar
- Almeida AP, Baptista SS, Sousa CA, Novo MT, Ramos HC, Panella NA, Godsey M, Simões MJ, Anselmo ML, Komar N, Mitchell CJ, Ribeiro H: Bioecology and vectorial capacity of Aedes albopictus (Diptera: Culicidae) in Macao, China, in relation to dengue virus transmission. J Med Entomol. 2005, 42 (3): 419-28. 10.1603/0022-2585(2005)042[0419:BAVCOA]2.0.CO;2.View ArticlePubMedGoogle Scholar
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