Effectiveness of autodissemination stations containing pyriproxyfen in reducing immature Aedes albopictus populations
© The Author(s). 2017
Received: 20 July 2016
Accepted: 14 February 2017
Published: 9 March 2017
Aedes albopictus, the Asian tiger mosquito, is an aggressive, highly anthropophilic, day-biting mosquito with an expanding geographic range. Suppression of Ae. albopictus is difficult because of the abundance and prevalence of larval habitats within peridomestic environments, particularly cryptic habitats such as corrugated extension spouts, fence post openings, discarded food containers, etc. Because of the challenges of eliminating or treating larval habitats of this species, we tested an autodissemination concept to contaminate these habitats with the insect growth regulator pyriproxyfen.
Our study was conducted in the City of Trenton (Mercer County), New Jersey, USA (40°12′N, 74°44′W). We selected six hot spots, where five or more Ae. albopictus males or females were collected based on weekly trap surveillance. A trapping unit was a city block, approximately 0.8 ha (hot spot), where we deployed 26 to 28 autodissemination stations per treatment plot. To gauge efficacy, we deployed BGS traps, oviposition cups, and sentinel cups in treatment and control locations.
We found a significant reduction in eggs (P < 0.0001) and larval populations (P < 0.0001) as a result of treatment. Pupal mortality, as determined through bioassays, was also significantly higher in the treatment sites (P < 0.0001).
Our results clearly show the potential and unique use of the autodissemination stations to control immature Ae. albopictus in urban areas. Penetration of larvicides with existing methods are difficult to reach cryptic habitats, but the autodissemination approach, which exploits the oviposition behavior of the target pest, can be integrated into intervention programs. New tools are urgently needed to curb the expansion and public health implications of Ae. albopictus and other container-inhabiting species.
KeywordsAsian tiger mosquito Autodissemination station Pyriproxyfen Egg surveillance Adult surveillance
Recent outbreaks of chikungunya (CHIKV) and Zika (ZIKV) virus infections have stimulated increased interest in management strategies for vector species such as Aedes aegypti (L.) and Aedes albopictus (Skuse) [1–4]. The latter species has been reported as the sole vector of CHIKV in explosive epidemics on the islands of La Réunion and Mauritius , and a potential secondary vector of ZIKV . The CHIKV outbreak was caused by a new variant of the virus [Reunion Island CHIKV isolates (CHIKV 226OPY1)] with a single adaptive mutation, an amino acid change from alanine to valine in the E1 glycoprotein which increased the infection and dissemination of the virus in Ae. albopictus [5–7]. Since there are no vaccines or specific antiviral treatments available for CHIKV or ZIKV, Ae. albopictus surveillance and control is critical for the prevention of outbreaks.
Aedes albopictus is a container-inhabiting mosquito, which in its native range of southeastern and eastern Asia, oviposits in tree holes, bamboo nodes, and a variety of artificial containers . The adaptation of this species to exploit artificial containers, in combination with its affinity for urban environments where containers such as buckets and tires are abundant, poses a challenge to control this species [9–12]. Aedes albopictus also exhibits skip oviposition, where the species may oviposit eggs from the same batch in multiple containers [13, 14]. Most water-holding containers within the peridomestic environment are suitable larval habitats for Ae. albopictus [9, 15]. Previous studies have reported that corrugated extension spouts routinely contain larvae and pupae of Ae. albopictus in residential backyards of urban and suburban areas of northeastern USA .
Suppressing populations of Aedes albopictus has been traditionally more difficult than Ae. aegypti, in part because of differences in their oviposition preferences [17, 18]. Aedes albopictus prefer numerous smaller habitats, which is much different than wetland mosquito species that develop in habitats that are large and predictable . We have also shown in previous studies  that immature Ae. albopictus numbers were higher in cryptic corrugated extension spouts (containers positioned on the ground horizontally) than open containers (containers positioned on the ground vertically) during the peak season (August). Because of the affinity of Ae. albopictus for cryptic habitats, area-wide larviciding using backpacks  or truck-mounted sprayers are not optimal control measures due to the poor penetration of pesticides into these habitats [16, 20]. Door-to-door source reduction campaigns are also labor and time intensive, often leading to ineffective control outcomes [21–23]. The autodissemination approach may be a means of insecticide delivery into cryptic habitats that are otherwise hard to treat by conventional methods.
Autodissemination is a ‘pull’ (attraction and adhesion) and ‘push’ (dispersal and transfer) technology which allows mosquito control professionals to treat larval habitats in a timely and economical fashion. Briefly, autodissemination is a pest management method in which insects contaminated with an insecticide, transfer lethal concentrations horizontally or vertically to other insects via mating, oviposition, aggregation and other behaviors [24–27]. The success of autodissemination stations depends on three criteria: (i) attraction of mosquitoes to the stations; (ii) transfer of chemicals to the mosquitoes; and (iii) dissemination of chemicals to target habitats . Gaugler et al.  achieved all three conditions with their 2012 design during semi-field trials and successfully contaminated containers using mosquitoes as the vehicles. A novel ovitrap was developed based on pull and push strategy to control Ae. aegypti by Snetselar et al. , using a combination of attractant media, Beauveria bassina (an entomopathogenic fungi), and pyriproxyfen (a biopesticide). Researchers achieved 100% larval mortality in the laboratory; however, the study did not include any field trials. The most commonly used chemical in autodissemination stations has been the insect growth regulator (IGR) pyriproxyfen, which does not cause immediate mortality or impair adult activity , but is effective on immature mosquitoes at extraordinarily low concentrations (LC50 in Ae. albopictus 0.012 ppb and Ae. aegypti is 0.023 ppb) [24, 30]. This approach seems to be a feasible strategy based on Ae. albopictus and Ae. aegypti skip-oviposition behavior, facilitated by distributing eggs from a single gonothrophic cycle . Devine at al.  achieved 42–98% Ae. aegypti adult emergence inhibition by using pyriproxyfen-treated stations in Peru . Autodissemination has also been tested by different groups in small scale field experiments against two dengue vectors [10, 26, 32]. Recently, Abad-Franch et al.  evaluated the efficacy of emulsifiable pyriproxyfen stations in a tropical neighborhood by monitoring Ae. aegypti, Ae. albopictus and Culex spp. populations. This approach resulted in a 10-fold decrease in adult mosquito emergence. However, large scale autodissemination applications need further investigation. A novel approach, “Auto-Dissemination Augmented by Males” (ADAM), has proven that pyriproxyfen-dusted males were able to transmit lethal amounts of insecticide to oviposition sites, resulting in reduced adult populations of Ae. albopictus . Most studies have primarily shown reduction of immature and adult populations caused by pupal mortality, and reduced egg number of Ae. aegypti females exposed to sub-lethal doses of pyriproxyfen [34, 35]. In the current study, we evaluated the effects of deployed autodissemination stations in Ae. albopictus hot spots (localized areas with high numbers of adults) through monitoring of eggs, larvae, pupae and adult populations in a temperate urban area, Trenton, New Jersey, USA.
Study site description
Our study was conducted in the City of Trenton (Mercer County), New Jersey, USA (40°12′N, 74°44′W). Trenton sites were urban residential neighborhoods: South Olden (40°22′N, 74°73′W) was 48.6 ha consisting of 24 city blocks, each containing a residential street on all four sides and divided lengthwise by a drivable alley. South Olden consisted of 1250 parcels (i.e. house with surrounding yard), most often built as adjoining row homes or duplexes . Parcel sizes were approximately 200 m2. We have continuous Ae. albopictus surveillance data between 2008 and 2014 from this study site, demonstrating high Ae. albopictus populations [9, 22, 37].
Identification of Ae. albopictus hot spots
To reduce the immigration from outside the study sites, we conducted area-wide larviciding with a water-dispersible granular (WDG) formulation of Bacillus thuringienis var. israelensis (Bti; VectoBac® WDG, Valent BioSciences Corp., Libertyville, IL, USA) prior to autodissemination station deployment. This was conducted within the barrier zone created around the sites, but excluded the experimental sites. We used a CSM2 Mist Sprayer (Buffalo Turbine, Springville, NY, USA) , for the Bti applications, and conducted the first application on 27 July between 01:00 and 06:00 h. Following the initial larviciding, ultra-low volume (ULV) adulticide applications using DUETTM Dual-action Adulticide (Clarke Mosquito Control, Roselle, IL, USA) were conducted each week around the treatment and control sites until 26 September. A Cougar® (Clarke Mosquito Control, Roselle, IL, USA) cold aerosol ULV generator was used during all adulticide applications. Truck-mounted adulticide applications were conducted at night using a single vehicle to drive buffer areas including all available roads and alleys to provide maximum coverage. Each application took about 2 h to complete and was conducted between 01:00 and 4:00 h .
Black 360 ml oviposition cups (SpringStar, Inc., Woodinville, WA, USA)] were zip-tied to a fence at ground level to reduce disturbance. The oviposition cups were filled with 300 ml of tap water, and seed germination paper was used to cover the inside surface acting as oviposition substrate. Two small holes were pre-drilled above the water line to prevent them from overflowing after a rain event. We deployed 30 oviposition cups (5 × 3 treatment sites, 5 × 3 control sites, Fig. 1). The oviposition papers were changed at 3 to 5 day intervals to coincide with adult trapping. Positive egg papers were subsampled (5 egg papers per week) and submersed in dechlorinated water in the laboratory. Because Aedes japonicus japonicus (Theobald) and Aedes triseriatus (Say) eggs other two mosquito species commonly found in containers in New Jersey , are very similar to Ae. albopictus eggs, larval food was provided until immatures reached third-instar stage and identified. Over 98% of the larvae were identified as Ae. albopictus .
Larval surveillance and pupal mortality
Initially, 10 sentinel cups were placed at each hot spot and filled with 250 ml of dechlorinated tap water (Fig. 1). To improve stability during inclement weather or other disturbances, we developed an anchoring system using a similar black cup with a hole in the bottom where a 20 cm galvanized spike could be driven securely into the ground, creating a nest to hold the sentinel cups in place. The sentinel cups were sampled at 2 week intervals as fresh water are less preferred in comparison to natural habitats . Longer exposure provides the opportunity to deliver sufficient pyriproxyfen into cups through multiple visits of mosquitoes, which compensates for absorption of pyriproxyfen in sentinel cups. The original cups were left in the field for the entirety of the study. If a collected cup was below 250 ml or empty, additional dechlorinated water was added as needed, agitated in the cup, and poured into the new sample cup for bioassays. After sampling, the sentinel cups were replenished with 250 ml of water and returned to their corresponding locations. The control sites were always sampled first and placed in an enclosed container inside the cab of the vehicle, while the treatment samples were placed in the back of the vehicle upon completion. Gloves were changed for each sampling to prevent contamination. Each sentinel cup from the control and treatment hot spots were observed, and the number of larvae, pupae and pupal cases in each container was recorded. After counting immature mosquito populations, sentinel cups were filtered to remove debris, organic materials and immature mosquitoes. A 50 ml water sample was taken from the sentinel cups for pyriproxyfen residue analysis. The residue analysis of pyriproxyfen was carried out at Golden Pacific Laboratories (CA, USA) using a liquid-mass-mass spectrophotometry (LC-MS-MS) analysis as described by Wang et al. . For the analysis, sample thawed, diluted 1:1 with acetonitrile and aliquot was passed through 0.45 μm filters. Primary and secondary ion pairs was monitored for the analysis using an AB Sciex API 5000 mass spectrophotometer. Each set of samples contained water blank and positive controls fortified at the limit of quantification and at a higher level to bracket expected sample concentrations. With the remaining 200 ml, we conducted larval bioassay by inoculating 20 third instars at 26 ± 1 °C and 16:8 L:D photoperiod. For colony mosquitoes, larval food (brewer’s yeast, 30 mg/l) was provided twice a week. Larval and pupal mortality and adult emergence were recorded to estimate efficacy. For negative control, three cups were set up using distilled water and 20 larvae per bioassay. Mosquito colony information and maintenance are described in detail elsewhere . Briefly, larvae obtained for bioassays were from an Ae. albopictus colony established from the eggs of field populations collected in Mercer County, New Jersey, USA between 2008 and 2010 . Restrained guinea pigs were used to blood-fed the females (Rutgers University Animal Use Protocol No. 86–129) and eggs were collected on seed germination paper and stored at 26 ± 1 °C. Third stage larvae were used in all bioassays.
We used BGS traps for adult mosquito sampling [37, 40], baited with BG lures (Biogents AG, Regensburg, Germany). Details of surveillance protocols are outlined elsewhere ; in short, trapping sites were chosen by overlaying a grid of 175 m intervals based on Ae. albopictus flight range  and available traps and personnel. The Fishnet tool within ArcGIS Desktop 9.2 (Environmental Systems Research Institute, Redlands, CA, USA) was used to determine potential trapping sites. The 175 m grid resulted in 16 traps within the study site (Fig. 1). Permission to place BGS traps was acquired from property owners. Traps were deployed in the field continuously for 24 h once per week during the mosquito season, and all mosquitoes were identified to species and gender . Trapping started 14 May and ended 12 November when no mosquitoes were collected for 2 to 3 weeks.
The effect of placement of autodissemination station in the treatment sites was investigated by comparing the number of eggs collected from oviposition cups at the treatment sites (HA 1-3) to the number of eggs collected from oviposition cups at the control sites (HC 1-3) using negative binomial regression (PROC GENMOD, SAS version 9.3; SAS Institute 2011), with treatment, week, and treatment*week as predictors, where treatment is an indicator variable (1 if site contained Autodissemination station, 0 otherwise). Since the number of days oviposition cups remained in the field varied, the natural log of the number of days a oviposition cup remained in the field was used as an offset. The number of larvae occurring in sentinel cups collected from the control and treatment sites were compared by negative binomial regression in SAS using the default log link. None of the sentinel cups contained larvae in the second week, therefore, data from this week were not included in the analysis. The model used treatment as a predictor for larval data showed signs of misfit, so instead the model was fitted using site, week, and site*week as predictors. Comparison of the mean number of larvae collected over the three treatment sites and three control sites was accomplished using a linear contrast. The mean pupal mortality from the laboratory bioassays using water samples collected from sentinel cups from all sites were compared by negative binomial regression using the default log link. The P-values between comparisons were adjusted using Holm’s test, which adjusts the calculation of probability in line with the number of comparisons made to avoid type I errors . No offset was used since the same initial number of laboratory colony larvae (20) used to inoculate all samples. Negative binomial regression was utilized in lieu of logistic regression because negative binomial regression is better suited for data containing a large number of zero values.
Adult mosquito numbers were compared using piecewise negative binomial regression in SAS . Separate slopes were fit for the before-treatment (12 June to 1 August) and after-treatment (5 August to 30 September) periods to examine whether the slope changed after placement of autodissemination stations in the treatment sites. We tested for the main effects of treatment, and of time in the before-treatment and time in the after-treatment periods, and for the interactions of treatment and time in both periods. These interactions were included to determine if the slopes differed between the treatment (HA1-3) and control (HC1-3) in either before or after-treatment periods. Time was treated as a continuous variable for the analysis of adult and egg data and as a categorical variable for the analysis of larval and pupal data.
Larval populations and pupal mortality
Number of larvae and dead pupae by site with least square means and 95% confidence intervals for treatments control
LS mean (95%CI)
LS mean (95% CI)
Aedes albopictus adult populations
We assessed autodissemination as a means of reducing Ae. albopictus populations in hot spots, which was ultimately an attempt to manage this species area-wide. It has been shown that by targeting immature Ae. albopictus populations in hot spots, it is possible to achieve area-wide control . Studies have demonstrated the efficacy of an autodissemination approach to reduce egg numbers in Aedes species in cages, rooms, and small scale field settings [10, 26, 32]. Our study targeted an area as large as 0.8 ha in an urban habitat and evaluated the impact of the stations on Ae. albopictus populations. We found egg and larval populations were reduced in autodissemination sites. These results were supported by higher pupal mortality during laboratory bioassays. There was no significant effect on the number of adult populations following autodissemination deployment; however, the overall trend in treatment and control sites were similar, indicating that adult numbers increased and decreased during repeated measurements of adult populations, mostly because our calculations were based on six traps (7 weeks). Even though we had long periods of follow-up with repeated measurements of adults using BGS traps to assess the effect of autodissemination station deployment, because of large variations in adult populations between sampling sites and across different sampling periods, the true effect on adults was not clear . In addition, given the unique biology of container-inhabiting Aedes mosquitoes, we cannot discount recurring immigration into our sampling sites during the course of these studies. While the immigration of adults limited our ability to detect a decline in adult populations within the hot spots, these mosquitoes likely helped distribute pyriproxyfen to larval containers. This is supported by the detection of pyriproxyfen residue in treatment site sentinel cups, pupal mortality, and the presence of significantly less eggs and larvae compared to control site sentinel cups . Although adult numbers were not significantly reduced, we recorded lower egg numbers from the treatment sites, which demonstrated that the stations were effectively contaminating adults and impacting their fecundity, leading to less egg collection from treatment sites. A previous study reported that pyriproxyfen contamination inhibits Ae. albopictus egg production . In summary, adult movement makes it difficult to interpret the findings although we tried to reduce immigration by weekly ULV adulticiding events, however, measurements of all three immature stages support our main conclusion that autodissemination stations reduced Ae. albopictus populations in the sites. Our results on adult populations were inconclusive due to the low number of BGS traps deployed and immigration. The availability of additional adult data may still not capture the difference between treatment and control sites because adult immigration from adjacent sites was reduced but not eliminated by weekly adulticiding. Despite these limitations, the presence of less eggs and larvae could only be explained by sub-lethal exposure of the females to pyriproxyfen. For future studies, we will increase the number of treatment and control sites along with the number of BGS traps per site to improve adult surveillance. Barrier treatments using backpack and hand sprayers on vegetation in the buffer areas, will be applied as an alternative to ULV adulticiding to further decrease immigration into the study sites.
One of the strengths of the experimental design for this study was to investigate the effect of our treatment on each life stage of the mosquito. In addition to monitoring adult populations, we collected larvae and water from sentinel cups for bioassays. The water was used to challenge laboratory colonies which allowed us to determine the effects of pyriproxyfen on pupal stages. We also used residue data to confirm whether or not pyriproxyfen is disseminating in the field. Correlation between the mortality in field collected cups and the residue analysis of samples is challenging as the amount of organic content varies in field collected samples. The organic content degrades and adsorbs pyriproxyfen, which makes it harder to extract from the water phasic substrates using organic solvents. Most of the time, this process resulted in loss of pyriproxyfen and that produced variations in the results . Whereas in the larval bioassay, larvae are continuously exposed to the same water for 2–3 weeks, and organic contents degrade over time, which provides regular supply as similar to slow release granule formulations of insecticides.
The autodissemination approach presented here shows promise to be a new tool for the control of a mosquito responsible for reducing quality of life and initiating disease outbreaks. Aedes albopictus is difficult to control and integrated abatement methods must be used to suppress the populations [15, 17, 19, 22, 37, 50]. Autodissemination station deployments can be incorporated with existing integrated mosquito management programs to increase the effectiveness while reducing time, cost, and effort spent for methods such as door-to-door source reduction. Target containers (plant pot saucers, recycle bins, used tires, etc.) are usually located within private residential parcels which are sometimes  inaccessible to mosquito control personnel. Even if inspectors can gain access to the parcels and treat all containers with larvicides, new ones frequently appear . This approach is labor- and cost-intensive, while the present study indicates that this exceptionally low risk and relatively inexpensive ($ 4.5 to $ 100 per kg) active ingredient, pyriproxyfen, offers promise. Pyriproxyfen is certainly deserving of further investigations, with the possible outcome of a modest label modification.
Another challenge with Ae. albopictus is its use of cryptic habitats for larval development. Unlu et al.  reported a dominant representation of Ae. albopictus in corrugated extension spouts . Because container-inhabiting Aedes species prefer cryptic larval habitats, which are difficult to reach by traditional mosquito control techniques [9, 52], the autodissemination approach may be a uniquely useful tool because it exploits female oviposition behavior to deliver a toxicant to these habitats . Container-inhabiting Aedes species also exhibit skip-oviposition and oviposit in a wide number and variety of artificial containers within peridomestic habitats [13, 53]. The autodissemination approach provides a unique opportunity for vector control operations to utilize skip-oviposition to their advantage globally.
Despite ongoing advances in the biology and ecology of Ae. albopictus and other container-inhabiting mosquito species, effective vector control remains as the primary option for the protection of human health and comfort. Aedes albopictus is also poised to expand its range in the next few decades; primarily because of warmer winter temperatures . The lack of effective area-wide control measures will present a major challenge to mosquito control agencies tasked with the protection of public health. Better planning and integration of new control products and methods, such as the autodissemination approach, will be the key for successful intervention campaigns.
- BGS traps:
BG sentinel traps
Bacillus thuringienis var. israelensis
We thank Nicholas Indelicato, Walt Voorhees, William Cook, Madison Rittley, Michael Scozzaro, Dan Scozzoro, Michael Milewski and Kshitij Chandel who assisted with surveillance, bioassays and adulticiding. Special thanks to Michael Banfield, SpringStar Inc.
This work was funded in part small business innovative Research (SBIR) program collaboration Agreement between NIH National Institutes of Health, Rutgers University and SpringStar Inc. entitled “Development of an auto-dissemination station for control of mosquito vectors.”
Availability of data and materials
All the datasets analyzed during this study are included within the article and in the Additional file 1.
RG, DS, YW, AF and IU conceived the study and coordinated its implementation. RG, DS, YW, AF and IU participated in the experimental design. Statistical analysis performed by KK and IU. DS, YW and IU performed the experiments. IU and KK drafted the manuscript which was critically revised by RG, DS, YW and AF. All authors read and approved the final version of the manuscript.
The authors declare that they have no competing interests.
Consent for publication
Ethics approval and consent to participate
All studies were conducted within the jurisdictions of the authors’ respective governance domain by professional mosquito control personnel. All entomological surveys and collections made on private lands or in private residences were conducted after acquisition of oral or written consent from residents. No specific permits were required for the mosquito collections. These studies did not involve endangered or protected species.
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
- CDC CfdcaP (2016) Chikungunya in the Caribbean. https://wwwnc.cdc.gov/travel/notices/watch/chikungunya-caribbean. Accessed 1 Feb 2017.
- Delatte H, Dehecq JS, Thiria J, Domerg C, Paupy C, Fontenille D. Geographic distribution and developmental sites of Aedes albopictus (Diptera: Culicidae) during a Chikungunya epidemic event. Vector Borne Zoonotic Dis. 2008;8:25–34.View ArticlePubMedGoogle Scholar
- LaDeau SL, Allan BF, Leisnham PT, Levy MZ. The ecological foundations of transmission potential and vector-borne disease in urban landscapes. Funct Ecol. 2015;29:889–901.View ArticlePubMedPubMed CentralGoogle Scholar
- Campos GS, Bandeira AC, Sardi SI. Zika virus outbreak, Bahia, Brazil. Emerging Infect Dis. 2015;21:1885.View ArticlePubMedPubMed CentralGoogle Scholar
- Ruiz-Moreno D, Vargas IS, Olson KE, Harrington LC. Modeling dynamic introduction of Chikungunya virus in the United States. PLoS Negl Trop Dis. 2012;6:e1918.View ArticlePubMedPubMed CentralGoogle Scholar
- Schuffenecker I, Iteman I, Michault A, Murri S, Frangeul L, Vaney MC, et al. Genome microevolution of chikungunya viruses causing the Indian Ocean outbreak. PLoS Med.2006;3:e263.
- Tsetsarkin KA, Vanlandingham DL, McGee CE, Higgs S. A single mutation in chikungunya virus affects vector specificity and epidemic potential. PLoS Pathog. 2007;3(12):e201.View ArticlePubMedPubMed CentralGoogle Scholar
- Preechaporn W, Jaroensutasinee M, Jaroensutasinee K. The larval ecology of Aedes aegypti and Ae. albopictus in three topographical areas of Southern Thailand. Dengue Bull. 2006;30:204.Google Scholar
- Unlu I, Farajollahi A, Strickman D, Fonseca DM. Crouching tiger, hidden trouble: Urban sources of Aedes albopictus (Diptera: Culicidae) refractory to source-reduction. PLoS One. 2013;8:e77999.View ArticlePubMedPubMed CentralGoogle Scholar
- Caputo B, Ienco A, Cianci D, Pombi M, Petrarca V, Baseggio A, et al. Auto-dissemination approach: a novel concept to fight Aedes albopictus in urban areas. PLoS Negl Trop Dis. 2012;6:e1793.
- Bartlett-Healy K, Unlu I, Obenauer P, Hughes T, Healy S, Crepeau T, et al. Larval mosquito habitat utilization and community dynamics of Aedes albopictus and Aedes japonicus (Diptera: Culicidae). J Med Entomol. 2012;49:813–24.
- Chan K, Ho B, Chan Y. Aedes aegypti (L.) and Aedes albopictus (Skuse) in Singapore City: 2. Larval habitats*. Bull WHO. 1971;44:629.PubMedPubMed CentralGoogle Scholar
- Richards SL, Apperson CS, Ghosh SK, Cheshire HM, Zeichner BC. Spatial analysis of Aedes albopictus (Diptera: Culicidae) oviposition in suburban neighborhoods of a Piedmont community in North Carolina. J Med Entomol. 2006;43:976–89.View ArticlePubMedGoogle Scholar
- Fonseca D, Kaplan L, Heiry R, Strickman D. Density-dependent oviposition by female Aedes albopictus (Diptera: Culicidae) spreads eggs among containers during the summer but accumulates them in the fall. J Med Entomol. 2015;52:705–12.View ArticlePubMedGoogle Scholar
- Richards SL, Ghosh SK, Zeichner BC, Apperson CS. Impact of source reduction on the spatial distribution of larvae and pupae of Aedes albopictus (Diptera: Culicidae) in suburban neighborhoods of a Piedmont community in North Carolina. J Med Entomol. 2008;45:617–28.View ArticlePubMedPubMed CentralGoogle Scholar
- Unlu I, Faraji A, Indelicato N, Fonseca DM. The hidden world of Asian tiger mosquitoes: immature Aedes albopictus (Skuse) dominate in rainwater corrugated extension spouts. Trans R Soc Trop Med Hyg. 2014;108:699–705. doi:10.1093/trstmh/tru1139.View ArticlePubMedGoogle Scholar
- Wheeler AS, Petrie WD, Malone D, Allen F. Introduction, control and spread of Aedes albopictus on Grand Cayman Island, 1997-2001. J Am Mosq Control Assoc. 2009;25:251–9.View ArticlePubMedGoogle Scholar
- Estrada-Franco JG, Craig GB. Biology, disease relationships, and control of Aedes albopictus. Pan American Health Organization Pan American Sanitary Bureau, Regional Office of the World Health Organization. 1995.
- Sun D, Williges E, Unlu I, Healy S, Williams GM, Obenauer P, et al. Taming a tiger in the city: comparison of motorized backpack applications and source reduction against the Asian tiger mosquito, Aedes albopictus. J Am Mosq Control Assoc. 2014;30:99–105.
- Williams GM, Faraji A, Unlu I, Healy SP, Farooq M, Gaugler R, et al. Area-wide ground applications of Bacillus thuringiensis var. israelensis for the control of Aedes albopictus in residential neighborhoods: from optimization to operation. PLoS One. 2014;9:e110035.
- Zhou YB, Zhao TY, Leng PE. Evaluation on the control efficacy of source reduction to Aedes albopictus in Shanghai, China. Chin J Vec Biol Contr. 2009;20:3–6.Google Scholar
- Fonseca DM, Unlu I, Crepeau T, Farajollahi A, Healy SP, Bartlett‐Healy K, et al. Area-wide management of Aedes albopictus. Part 2: gauging the efficacy of traditional integrated pest control measures against urban container mosquitoes. Pest Manage Sci. 2013;69:1351–61.
- Bartlett-Healy K, Hamilton G, Healy S, Crepeau T, Unlu I, Farajollahi A, et al. Source reduction behavior as an independent measurement of the impact of a public health education campaign in an integrated vector management program for the Asian tiger mosquito. Int J Env Res Public Health. 2011;8:1358–67.
- Gaugler R, Suman D, Wang Y. An autodissemination station for the transfer of an insect growth regulator to mosquito oviposition sites. Med Vet Entomol. 2012;26:37–45.View ArticlePubMedGoogle Scholar
- Wang Y, Suman DS, Bertrand J, Dong L, Gaugler R. Dual treatment autodissemination station with enhanced transfer of an insect growth regulator to mosquito oviposition sites. Pest Manage Sci. 2014;70:1299–304.View ArticleGoogle Scholar
- Mains JW, Brelsfoard CL, Dobson SL. Male mosquitoes as vehicles for insecticide. PLoS Negl Trop Dis. 2015;9:e0003406.View ArticlePubMedPubMed CentralGoogle Scholar
- Geden CJ, Devine GJ. Pyriproxyfen and house flies (Diptera: Muscidae): effects of direct exposure and autodissemination to larval habitats. J Med Entomol. 2012;49:606–13.View ArticlePubMedGoogle Scholar
- Snetselaar J, Andriessen R, Suer RA, Osinga AJ, Knols B, Farenhorst M. Development and evaluation of a novel contamination device that targets multiple life-stages of Aedes aegypti. Parasit Vectors. 2014;7:1.View ArticleGoogle Scholar
- Kawada H, Shono Y, Ito T, Abe Y. Laboratory evaluation of insect growth regulators against several species of anopheline mosquitoes. Jap J Sanit Zool. 1993;44:349.View ArticleGoogle Scholar
- Itoh T. Utilization of blood fed females of Aedes aegypti as a vehicle for the transfer of the insect growth regulator, pyriproxyfen, to larval habitats. Tropical Medicine. 1995;36:243–8.Google Scholar
- Trexler JD, Apperson CS, Schal C. Laboratory and field evaluations of oviposition responses of Aedes albopictus and Aedes triseriatus (Diptera: Culicidae) to oak leaf infusions. J Med Entomol. 1998;35:967–76.View ArticlePubMedGoogle Scholar
- Devine GJ, Perea EZ, Killeen GF, Stancil JD, Clark SJ, Morrison AC. Using adult mosquitoes to transfer insecticides to Aedes aegypti larval habitats. Proc Natl Acad Sci U S A. 2009;106:11530–4.View ArticlePubMedPubMed CentralGoogle Scholar
- Abad-Franch F, Zamora-Perea E, Ferraz G, Padilla-Torres SD, Luz SL. Mosquito-disseminated pyriproxyfen yields igh breeding-site coverage and boosts juvenile mosquito mortality at the neighborhood scale. PLoS Negl Trop Dis. 2015;9:e0003702.View ArticlePubMedPubMed CentralGoogle Scholar
- Dash AP, Ranjit MR. Comparative efficacy of aphid extracts and some juvenoids against the development of mosquitoes. J Am Mosq Control Assoc. 1992;8:247–51.PubMedGoogle Scholar
- Ponlawat A, Fansiri T, Kurusarttra S, Pongsiri A, McCardle PW, Evans BP, Richardson JH. Development and evaluation of a pyriproxyfen-treated device to control the dengue vector, Aedes aegypti (L.) (Diptera:Culicidae). Southeast Asian J Trop Med Public Health. 2013;44:167–78.PubMedGoogle Scholar
- Unlu I, Farajollahi A, Healy SP, Crepeau T, Bartlett‐Healy K, Williges E, et al. Area-wide management of Aedes albopictus: choice of study sites based on geospatial characteristics, socioeconomic factors and mosquito populations. Pest Manage Sci.2011;67:965–74.
- Unlu I, Farajollahi A, Ilia R, Crepeau TN, Daniel S, Gaugler R. Differences in male-female ratios of Aedes albopictus (Diptera: Culicidae) following ultra-low volume adulticide applications. Acta Trop. 2014;137:201–5.View ArticlePubMedGoogle Scholar
- Carrieri M, Bellini R, Maccaferri S, Gallo L, Maini S, Celli G. Tolerance thresholds for Aedes albopictus and Aedes caspius in Italian urban areas. J Am Mosq Control Assoc. 2008;24:377–86.View ArticlePubMedGoogle Scholar
- Read NR, Rooker JR, Gathman JP. Public perception of mosquito annoyance measured by a survey and simultaneous mosquito sampling. J Am Mosq Control Assoc. 1994;10:79–87.PubMedGoogle Scholar
- Kroeckel U, Rose A, Eiras ÁE, Geier M. New tools for surveillance of adult yellow fever mosquitoes: comparison of trap catches with human landing rates in an urban environment. J Am Mosq Control Assoc. 2006;22:229–38.View ArticleGoogle Scholar
- Unlu I, Klingler K, Indelicato N, Faraji A, Strickman D. Suppression of Aedes albopictus, the Asian tiger mosquito, using a “hot spot” approach. Pest Manage Sci. 2015;72:1427–32.View ArticleGoogle Scholar
- Farajollahi A, Crans SC. A checklist of the mosquitoes of New Jersey with notes on established invasive species. J Am Mosq Control Assoc. 2012;28:237–9.View ArticlePubMedGoogle Scholar
- Stojanovich CJ. Illustrated key to common mosquitoes of northeastern North America. Cullom & Ghertner Nashville. 1961.
- Trexler JD, Apperson CS, Zurek L, Gemeno C, Schal C, Kaufman M, et al. Role of bacteria in mediating the oviposition responses of Aedes albopictus (Diptera: Culicidae). J Med Entomol. 2003;40:841–8.
- Holm S. A simple sequentially rejective multiple test procedure. Scand J Stat. 1979;65–70.
- Marsh LC, Cormier DR. Spline Regression Models, Quantitative Applications in the Social Sciences No. 137. Thousand Oaks: Sage; 2002.
- Wilson AL, Boelaert M, Kleinschmidt I, Pinder M, Scott TW, Tusting LS, Lindsay SW. Evidence-based vector control? Improving the quality of vector control trials. Trends Parasito. 2015;31:380–90.View ArticleGoogle Scholar
- Ohba SY, Ohashi K, Pujiyati E, Higa Y, Kawada H, Mito N, Takagi M. The effect of pyriproxyfen as a “population growth regulator” against Aedes albopictus under semi-field conditions. PLoS One. 2013;8:e67045.View ArticlePubMedPubMed CentralGoogle Scholar
- Picó Y, Fernández M, Ruiz MJ, Font G. Current trends in solid-phase-based extraction techniques for the determination of pesticides in food and environment. J Biochem Biophys Methods. 2007;70:117–31.View ArticlePubMedGoogle Scholar
- Farajollahi A, Healy SP, Unlu I, Gaugler R, Fonseca DM. Effectiveness of ultra-low volume nighttime applications of an adulticide against diurnal Aedes albopictus, a critical vector of dengue and chikungunya viruses. PLoS One. 2012;7:e49181.View ArticlePubMedPubMed CentralGoogle Scholar
- Hawley WA. The biology of Aedes albopictus. J Am Mosq Control Assoc. 1998;Suppl 1:1–39.Google Scholar
- Benedict MQ, Levine RS, Hawley WA, Lounibos LP. Spread of the tiger: global risk of invasion by the mosquito Aedes albopictus. Vector Borne Zoonotic Dis. 2007;7:76–85.View ArticlePubMedPubMed CentralGoogle Scholar
- Davis TJ, Kaufman PE, Tatem AJ, Hogsette JA, Kline DL. Development and evaluation of an attractive self-marking ovitrap to measure dispersal and determine skip oviposition in Aedes albopictus (Diptera: Culicidae) field populations. J Med Entomol. 2016;53(1):31-8.
- Rochlin I, Ninivaggi DV, Hutchinson ML, Farajollahi A. Climate change and range expansion of the Asian tiger mosquito (Aedes albopictus) in Northeastern USA: implications for public health practitioners. PLoS One. 2013;8:e60874.View ArticlePubMedPubMed CentralGoogle Scholar