Gel electrophoresis of Trypanosomes using ITS nested primers. Expected band sizes are 1207–1224 bp for T. brucei (T. b), 1422 bp for T. congolense kilifi (Tck) and 1413 bp savannah (Tcs)sub-types, and 611 bp for T. vivax (T. v) DNA samples. Test samples are labelled 21–28, negative control (-v) and 100 base pair ladders labelled 'M'. Sample numbered 24 & 26 are positive for T. vivax, with number 26 being a mixed infection with T. congolense savannah. No amplification on the negative controls.