Table 2 Gene specific primers used for RT-PCR expression profiling of I. ricinus peptidases

IrAE

^5'TCGGTGACGCTGAGAAGACTGAA^3'

^5'TAGATTATGCCCGATGACTGTTGG^3'

55°C

239 bp

IrCB

^5'CGACGGCGGATACCCGGCGGCAGC^3'

^5'CCGAGTACGTCACCGCTGTGGTGC^3'

60°C

360 bp

IrCL

^5'GTCACGCCCGTCAAGAACC^3'

^5'TGGCATCAATGGCAACAGAGA^3'

62°C

382 bp

IrCC

^5'CCACCCGGGACCCCTACAACACC^3'

^5'GGAAGCCGCCCTCGCAACC^3'

68°C

447 bp

IrCD

^5'GGACCCCGCCCCAAGACT^3'

^5'CGATGCCGCCGAACAGGA^3'

61°C

459 bp