Kinetics of LAMP for D. immitis microfilariae. (A) Amplification of target sequence with primer set monitored by real-time turbidimeter (turbidity at 650 nm). (B) Detection of reaction products by LAMP with genomic DNA from D. immitis microfilariae. 1 μl of reaction mixture was run on a 2% agarose gel. Amplified products were detectable with any samples containing genomic DNA corresponding to the amount of one or more microfilariae. Numbers on the left indicate migration of molecular weight marker (bp).